Biology and Biotechnology: Recent submissions
Now showing items 141-160 of 193
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The restriction endonuclease Tru9I is a useful tool to analyze telomere sequences separately from interstitial telomeric sequences in Arabidopsis thaliana
(Scientific Research Publishing, 2012-07)We have previously described a simple method to analyze the chromatin structure of Arabidopsis telomeres independently of that of Interstitial Telomeric Sequences (ITSs). By using this method, we found that, whereas ITSs ... -
Identification of ebs1, lsm6 and nup159 as suppressors of spt10 effects at ADH2 in Saccharomyces cerevisiae suggests post-transcriptional defects affect mRNA synthesis
(Scientific Research Publishing, 2012-07)Suppression of the effects of an spt10 mutation on ADH2 expression is a phenotype shared by a small number of genes whose protein products are either components of the CCR4-NOT complex required for mRNA deadenylation and ... -
How is the biological information arranged in genome?
(Scientific Research Publishing, 2012-07)The four nucleotides (bases), A. T. G and C were sophisticatedly arranged in the structural features in a single-strand of genomic DNA, 1) reverse-complement symmetry of base or base sequences, 2) bias of four bases, 3) ... -
Solving the independent set problem by sticker based DNA computers
(Scientific Research Publishing, 2012-04)In this paper, the sticker based DNA computing was used for solving the independent set problem. At first, solution space was constructed by using appropriate DNA memory complexes. We defined a new operation called “divide” ... -
Tandem repetitions in transcriptomes of some Solanaceae species
(Scientific Research Publishing, 2012-04)Characterization of occurrence, density and motif sequence of tandem repeats in the transcribed regions is helpful in understanding the functional significance of these repeats in the modern genomes. We analyzed tandem ... -
A multi-criteria decision-making approach for comparing sample preservation and DNA extraction methods from swine feces
(Scientific Research Publishing, 2012-04)Molecular microbiological methods, such as competetive PCR, real-time PCR, denaturing gradient gel electrophoresis (DGGE) and large-scale parallel-pyrosequencing, require the extraction of sufficient quantity of high quality ... -
Molecular cloning and characterization of fruit specific promoter from Cucumis sativus L.
(Scientific Research Publishing, 2012-04)The isolation and characterization of fruit specific promoters are critical for the manipulation of nutritional value and agronomic quality of fruits by genetic engineering and also opened a new era in edible vaccine ... -
Expression of the transcription factor Xvent-2 in Xenopus laevis embryogenesis
(Scientific Research Publishing, 2012-04)Till now the transcription factor Xvent-2 has been studied in Xenopus embryos only by the mRNA testing. We use immunochemical methods for testing of the Xvent-2 protein and gradient-centrifugation methods for estimation ... -
Modulation of gastric mucosal inflammatory responses to Helicobacter pylori by ghrelin: Role of cNOS-dependent IKK-β S-nitrosylation in the regulation of COX-2 activation
(Scientific Research Publishing, 2012-04)Disturbances in nitric oxide synthase (NOS) and cyclooxygenase (COX) isozyme systems, manifested by the excessive NO and prostaglandin (PGE2) generation, are well-recognized features of gastric mucosal inflammatory responses ... -
The ATPase activity of molecular chaperone HSP60 is inhibited by immunosuppressant mizoribine
(Scientific Research Publishing, 2012-04)The molecular chaperone HSP60 is a chaperonin homolog of GroEL. We had previously shown that the immunosuppressant mizoribine is bound directly to HSP60 and inhibited its chaperone activity. However, the inhibitory mechanisms ... -
Oxidized phospholipids modify plasminogen and apolipoprotein(a): Implications for vascular disease
(Scientific Research Publishing, 2012-04)Whereas the close structural homology between human plasminogen and apolipoprotein(a) has been known for a number of years only recent studies have revealed that both proteins carry linked oxidized phospholipids that may ... -
The effects of astragalus polysaccharide on zebrafish cell apoptosis and senescence
(Scientific Research Publishing, 2012-04)Astragalus polysaccharide (AP) is the extraction of astragalus, which is a plant used in traditional Chinese herb medicine and may increase an orgainism’s resistance to stress. Several earlier studies in vitro have indicated ... -
Somaclonal variations of Soybeans (Glycine Max. L. Merr) stimulated by drought stress based on random amplified polymorphic DNAs (RAPDs)
(Scientific Research Publishing, 2012-01)In soybeans, drought stress causes 50% yield losses. Breeding for drought tolerance in soybeans has been widely developed using various methods, among which is polyethylene glycol (PEG-6000) induction to simulate drought ... -
DOF transcription factors in developing peanut (Arachis hypogaea) seeds
(Scientific Research Publishing, 2012-01)DNA binding with one finger (DOF) transcription factors play important roles in storage material accumulation and morphogenesis of developing seeds. Oil and protein contents varied in different cultivars in important oil ... -
Intergenic subset organization within a set of geographically-defined viral sequences from the 2009 H1N1 influenza A pandemic
(Scientific Research Publishing, 2012-01)We report a bioinformatic analysis of the datasets of sequences of all ten genes from the 2009 H1N1 influenza A pandemic in the state of Wisconsin. The gene with the greatest summed information entropy was found to be the ... -
Comparative gene expression analysis in stems of Dolichos purpureus and Arabidopsis thaliana
(Scientific Research Publishing, 2012-01)The outermost layer epidermis of a plant stem plays an important role in protection and environment-sensing. The mechanisms of sensing and response to the environment through the stem epidermis remain unclear. Here we ... -
SPT5 affects the rate of mRNA degradation and physically interacts with CCR4 but does not control mRNA deadenylation
(Scientific Research Publishing, 2012-01)The CCR4-NOT complex has been shown to have multiple roles in mRNA metabolism, including that of transcriptional elongation, mRNA transport, and nuclear exosome function, but the primary function of CCR4 and CAF1 is in the ... -
Molecular docking investigation for Indonesian H274Y mutant neuraminidase type 1 with neuraminidase inhibitors
(Scientific Research Publishing, 2012-01)The aim of this study is to get insight the interaction between Indonesian H274Y mutant neuraminidase with four inhibitors. Not only to seek preferable inhibitor to be used, but also to investigate the interaction occurred, ... -
Molecular surveillance of dengue virus in Bahia State, Brazil
(Scientific Research Publishing, 2012-01)Dengue is an important emerging viruses, posing a threat to one-third of the global human population. In 2002, the introduction of DENV-3 in the state of Bahia produced massive epidemic (about 35,000 cases detected) and ... -
Effect of hypoxia and glutamine or glucose deprivation on the expression of retinoblastoma and retinoblastoma-related genes in ERN1 knockdown glioma U87 cell line
(Scientific Research Publishing, 2012-01)The expression of retinoblastoma and several retinoblastoma-related genes was studied in glioma cell line U87 and its subline with knockdown of ERN1 (endoplasmic reticulum—nuclei-1), the main endoplasmic reticulum stress ...
