The restriction endonuclease Tru9I is a useful tool to analyze telomere sequences separately from interstitial telomeric sequences in Arabidopsis thaliana
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Date
2012-07
Journal Title
Journal ISSN
Volume Title
Publisher
Scientific Research Publishing
Abstract
We have previously described a simple method to analyze the chromatin structure of Arabidopsis telomeres independently of that of Interstitial Telomeric Sequences (ITSs). By using this method, we found that, whereas ITSs are heterochromatic, Arabidopsis telomeres exhibit euchromatic features [1]. Some concerns have been recently raised about the accuracy of this procedure [2]. Here, we summarize these concerns and justify our experimental approaches and interpretation of results.
Description
Keywords
Arabidopsis thaliana, telomeres, ITSs, euchromatin, heterochromatin
Citation
American Journal of Molecular Biology, 2012, 2, 242-244