Biology and Biotechnology: Recent submissions
Now showing items 121-140 of 193
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GAPDH expression as a measurement of transfection efficiency for p16INK4a gene silencing (siRNA) in senescent human diploid fibroblasts
(Scientific Research Publishing, 2012-10)Human diploid fibroblasts (HDFs) undergo a limited number of cell divisions in culture. After certain population doublings, they reach a state of irreversible growth arrest known as replicative senescence. Senescent HDFs ... -
Genetic variation may play a crucial role in non-coding RNA biogenesis
(Scientific Research Publishing, 2012-10)Transcription, post-transcriptional modification, translation, post-translational modification, DNA replication, and signaling interaction of intra- and extra- cellular components are the relevant mechanisms in gene ... -
A simple and efficient seed-based approach to induce callus production from B73 maize genotype
(Scientific Research Publishing, 2012-10)The wild type maize genotype, B73, is not amenable for callus production and an efficient protocol for B73 maize callus induction has never been reported up-to-date. Scientific efforts in producing B73 maize callus using ... -
An engineered Phlebia radiata manganese peroxidase: expression, refolding, purification and preliminary characterization
(Scientific Research Publishing, 2012-10)Manganese peroxidases (MnPs) are interesting enzymes in protein engineering, aimed at maximizing industrial bioprocesses such as lignin degradation and biofuel production. cDNA of the secreted short-type of MnP from Phlebia ... -
Chimeric oncogenic interferon regulatory factor-2 (IRF-2): Degradation products are biologically active
(Scientific Research Publishing, 2012-10)Interferon Regulatory Factor-2 (IRF-2) belongs to IRF family, was identified as a mammalian transcrip-tion factor involved in Interferon beta (IFNβ) gene regulation. Besides that IRF-2 is involved in ... -
A silkworm hemolymph protein is a prophenoloxidase activation blocker
(Scientific Research Publishing, 2012-10)Melanization in insect hemolymph is triggered by the recognition of pathogen-associated molecular patterns via pattern recognition receptors. The signal transduction leads to the activation of the prophenoloxidase and hence ... -
Mutated elements of a complex promoter (Amh) can help to demonstrate the role of certain elements in controlling differential gene expression
(Springer Science+Business Media, 2012-10)Amh is a single copy gene which is expressed in different ways during mammalian development. Several potential promoter elements have been identified using physiological experimentation and on the basis of interspecific ... -
Biocatalytic production of chitosan polymers from shrimp shells, using a recombinant enzyme produced by pichia pastoris
(Scientific Research Publishing, 2012-10)Chitosan has a unique chemical structure with high charge density, reactive hydroxyl and amino groups, and extensive hydrogen bonding. Chitin deacetylase (EC 3.5.1.41) catalyzes the hydrolysis of the N-acetamido groups of ... -
Anaplerosis in cancer: Another step beyond the warburg effect
(Scientific Research Publishing, 2016-10)Biosynthesis is up-regulated in tumors and thus the demand for anabolic intermediates is increased. The metabolic routes providing the building blocks for macromolecules are thus a very attractive target as they are not ... -
SPOC domain of mint protein induces hematopoietic differentiation via Bmp4/Smad5 pathway
(Scientific Research Publishing, 2012-10)Mint is a newly identified molecule that mediates signal transduction and modulates chromatin repression. Mint family members contain a highly conserved C-terminus SPOC domain (SpenParalog and OrthologsC-terminal domain) ... -
Early expression of the tbx22 gene in zebrafish influences positioning of pharyngeal arch cartilages
(Scientific Research Publishing, 2012-10)Mutations in human TBX22 cause X-linked cleft palate with anklyoglossia syndrome. The two zebrafish tbx22 splice isoforms, tbx22-1 and tbx22-2, encode proteins of 444 and 400 amino acids, respectively. Zebrafish tbx22 mRNA ... -
Knockdown of Dock7 in vivo specifically affects myelination by Schwann cells and increases myelin thickness in sciatic nerves without affecting axon thickness
(Scientific Research Publishing, 2012-07)During development of the peripheral nervous system (PNS), Schwann cells (SCs) wrap individual axons to form myelin sheaths, which act as surrounding insulators and markedly enhance the propagation of the action potential. ... -
Applying Surface-Based DNA Computing for Solving the Dominating Set Problem
(Scientific Research Publishing, 2012-07)The surface-based DNA computing is one of the methods of DNA computing which uses DNA strands immobilized on a solid surface. In this paper, we applied surface-based DNA computing for solving the dominating set problem. ... -
A peptide of 17 aminoacids from the N-terminal region of maize plastidial transglutaminase is essential for chloroplast targeting
(Scientific Research Publishing, 2012-07)Transglutaminases (TGases, EC 2.3.2.13) catalyse posttranslational modification of proteins by establishing ε-(γ-glutamyl) links and covalent conjugation of polyamines. In plants, the functionality of these enzymes is ... -
Release of exosomes and microvesicles harbouring specific RNAs and glycosylphosphatidylinositol-anchored proteins from rat and human adipocytes is controlled by histone methylation
(Scientific Research Publishing, 2012-07)The transfer of proteins and nucleic acids from donor to acceptor cells via small membrane vesicles has been implicated with (patho)physiological consequences. Previously the upregulation of esterification and downregulation ... -
Marek’s disease virus challenge induced immune-related gene expression and chicken repeat 1 (CR1) methylation alterations in chickens
(Scientific Research Publishing, 2012-07)Marek’s disease virus (MDV) challenge induces lymphoma in susceptible chickens. Host genes, especially immune related genes, are activated by the virus. DNA methylation is an epigenetic mechanism that governs gene ... -
Suppression of Sup35 amyloid fibril formation by group II chaperonin from Thermoplasma acidophilum
(Scientific Research Publishing, 2012-07)The Group II chaperonin from Thermoplasma acidophilum was added to the in vitro amyloid fibrillation reaction of yeast Sup35NM protein to assess its effects. By measuring the formation of Sup35NM fibrils in real time using ... -
Effects of bar-transgenic rice on the intestinal microflora of the mice (Mus musculus)
(Scientific Research Publishing, 2012-07)Microbial molecular ecology approaches were used to the effects of Bar-transgenic rice on Intestinal Micro-flora of the Mice (Mus musculus). Kunming mice (Mus musculus) of 100 SPF-grade (20 g ± 2 g), half of which were ... -
The full length PtSRP (Pisolithus tinctorius symbiosis related protein) fungal mRNA encodes a potential marker of ectomycorrhiza formation
(Scientific Research Publishing, 2012-07)The Pisolithus tinctorius symbiosis related protein expressed sequence tag (EST PtSRP) was previously identified in the first hours of the interaction between the fungus Pisolithus tinctorius and sweet chestnut Castanea ... -
Expression and subcellular localization of a novel gene Bm-X of the silkworm, Bombyx mori
(Scientific Research Publishing, 2012-07)According to the large-scale sequencing of cDNA library from silkworm pupae, the cDNA of a novel gene with blank research background was identified and temporarily named Bm-X. The length of this cDNA is 778 bp. We obtained ...