Browsing Biology and Biotechnology by Title
Now showing items 71-90 of 193
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Effects of bar-transgenic rice on the intestinal microflora of the mice (Mus musculus)
(Scientific Research Publishing, 2012-07)Microbial molecular ecology approaches were used to the effects of Bar-transgenic rice on Intestinal Micro-flora of the Mice (Mus musculus). Kunming mice (Mus musculus) of 100 SPF-grade (20 g ± 2 g), half of which were ... -
Effects of fertilization and concentrate feeding on water quality and growth performance of Nile tilapia (Oreochromis niloticus) grown in concrete tanks
(African Science Publications, 2020-10-05)An experiment was conducted to compare the effects of fertilizer application alone, concentrate feeding alone and combination of fertilization and feeding on water quality parameters and growth performance of sex-reversed ... -
Effects of genetic and environmental factors and gene-environment interaction on expression variations of genes related to stroke in rat brain
(Scientific Research Publishing, 2011-07)To determine if genetic and environmental (dietary) factors and gene-environment interaction impact on the expression variation of genes related to stroke, we conducted microarray experiments using two homozygous rat strains ... -
Effects of microbial-fertilizers on the morphological parameters and biochemical content of Cow pea Vigna unguiculata (L.) Walp. – A biotechnological approach
(African Science Publications, 2021-01-01)The use of chemical fertilizers and agrochemicals has dangerous effects on both environment and human health. Frequently used chemical fertilizers to increase crop yields and reload soil nutrients have been extinguished ... -
The efficacy of biostimulants in the management of Agrobacterium tumefasciens the cause of crown gall disease of roses in Kericho, Kenya
(African Science Publications, 2021-04-05)Experiments were conducted at James Finlay Kenya, Tarakwet farm in Kericho county to test the efficacy of various biostimulants, i.e., biozyme 2.5 ml/L, hicure 2.5 ml/L, foltron 2.0 ml/L, codamine radicular 2.0 ml/L, alexin ... -
The efficacy of sterilizing agents, copper oxychloride, vegetable oil and agrowipe (botanic neem extract) against crown gall disease of roses in Kericho, Kenya
(African Science Publications, 2020-10-05)The experiments were conducted at James Finlay Kenya, flowers division Tarakwet farm in Kericho county from January 2016 to December 2016 to study the efficacy of copper oxychloride 1.0 g/L, hydrogen peroxide 1.0 ml/L, ... -
EGR1 is essential for transcriptional regulation of BMPR2
(Scientific Research Publishing, 2011-10)In this study, RLM-RACE was used to identify the transcriptional start site 387 bp upstream of the translational start. Evolutionarily conserved transcription factor binding sites were identified, and a series of luciferase ... -
An engineered Phlebia radiata manganese peroxidase: expression, refolding, purification and preliminary characterization
(Scientific Research Publishing, 2012-10)Manganese peroxidases (MnPs) are interesting enzymes in protein engineering, aimed at maximizing industrial bioprocesses such as lignin degradation and biofuel production. cDNA of the secreted short-type of MnP from Phlebia ... -
Evaluation of anti-inflammatory and healing activities of dichloromethane fraction of an ethanol extract of stem bark of Piliostigma reticulatum
(African Science Publications, 2020-07-09)The objective of this study was to evaluate the anti-inflammatory activity of the dichloromethane fraction of an ethanol extract of Piliostigma reticulatum, a medicinal plant from Côte d’Ivoire and widely used by traditional ... -
Evaluation of cellular and circulatory antioxidant- and glutathioneassociated enzymes in patients with acute coronary syndrome
(African Science Publications, 2021-04-05)Destabilization of atherosclerotic plaque in the coronary artery is the result of Oxidative Stress (OS) that damage the myocardial tissues in Acute Coronary Syndrome (ACS). This study investigated the activities of certain ... -
Explaining the benefits of human genome patent
(African Journal of Biological Sciences, 2019-04-12)Gene patenting has been facing backlash for decades now. Even Michael Crichton, science fiction novelist, as joined the bandwagon speaking against gene patent.1 Such hostility towards patents, the professional interests ... -
Expression Analysis of WRKY Transcription Factor Genes in Response to Abiotic Stresses in Horsegram (Macrotyloma uniflorum (Lam.) Verdc.)
(Scientific Research Publishing, 2016-10)Drought and salt stress are two major environmental constraints that limit the productivity of agriculture crops worldwide. WRKY transcription factors are the plant-specific transcription factors that regulate several ... -
Expression and subcellular localization of a novel gene Bm-X of the silkworm, Bombyx mori
(Scientific Research Publishing, 2012-07)According to the large-scale sequencing of cDNA library from silkworm pupae, the cDNA of a novel gene with blank research background was identified and temporarily named Bm-X. The length of this cDNA is 778 bp. We obtained ... -
Expression of the transcription factor Xvent-2 in Xenopus laevis embryogenesis
(Scientific Research Publishing, 2012-04)Till now the transcription factor Xvent-2 has been studied in Xenopus embryos only by the mRNA testing. We use immunochemical methods for testing of the Xvent-2 protein and gradient-centrifugation methods for estimation ... -
Extracellular Micro-RNAs in Health and Disease: Basic Science, Biogenesis and Release
(Scientific Research Publishing, 2016-01)Small non-protein coding micro-RNAs are regularly exported out of cells, both in health and disease. More than ninety percent of extracellular miRNAs are associated with lower-molecular-mass complexes bound to Argonaute ... -
Frequently cutting restriction enzymes: Clearing the fog to see the ends
(Scientific Research Publishing, 2013-01)Telomeres, which are found at the ends of eukaryotic chromosomes, are composed of tandem arrays of repetitive sequences and safeguard genomic stability. Previous studies have revealed that telomeric repeats are also present ... -
The full length PtSRP (Pisolithus tinctorius symbiosis related protein) fungal mRNA encodes a potential marker of ectomycorrhiza formation
(Scientific Research Publishing, 2012-07)The Pisolithus tinctorius symbiosis related protein expressed sequence tag (EST PtSRP) was previously identified in the first hours of the interaction between the fungus Pisolithus tinctorius and sweet chestnut Castanea ... -
Functional reconstruction of bovine P450scc steroidogenic system in Escherichia coli
(Scientific Research Publishing, 2013-10)Mammalian cytochrome P450scc enzyme system catalyzes the initial step in steroid hormone biosynthesis—cholesterol hydroxylation followed by cleavage of the side-chain to yield pregnenolone. This system consists of three ... -
GAPDH expression as a measurement of transfection efficiency for p16INK4a gene silencing (siRNA) in senescent human diploid fibroblasts
(Scientific Research Publishing, 2012-10)Human diploid fibroblasts (HDFs) undergo a limited number of cell divisions in culture. After certain population doublings, they reach a state of irreversible growth arrest known as replicative senescence. Senescent HDFs ... -
Gene expression profiling in soybean under aluminum stress: genes differentially expressed between Al-tolerant and Al-sensitive genotypes
(Scientific Research Publishing, 2011-10)It is well documented that aluminum (Al) toxicity is the most important constraint to crop production on acid soils and soybean is one of the most Al sensitive plant species. To advance our understanding of the molecular ...