• Login
    View Item 
    •   Repository
    • Open Access Articles
    • Open Access Journals
    • Biology and Biotechnology
    • View Item
    •   Repository
    • Open Access Articles
    • Open Access Journals
    • Biology and Biotechnology
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    EGR1 is essential for transcriptional regulation of BMPR2

    Thumbnail
    View/Open
    Full text (599.4Kb)
    Date
    2011-10
    Author
    Gaddipati, Radhika
    West, James D.
    Loyd, James E.
    Blackwell, Thomas
    Lane, Kirsten A.
    Lane, Nicole M.
    Lane, Kirk B.
    Metadata
    Show full item record
    Abstract
    In this study, RLM-RACE was used to identify the transcriptional start site 387 bp upstream of the translational start. Evolutionarily conserved transcription factor binding sites were identified, and a series of luciferase reporter constructs driven by BMPR2 promoter elements used to determine their functional relevance. We found the promoter area from 983 bp to 90 bp upstream of the transcriptional start gave maximal activity, greater than longer constructs, with an area between 570 bp and 290 bp upstream of the transcriptional start containing an important repressor element. To characterize this repressor, we used a combination of EMSA, mutation of the EGR1 binding site, transfection with EGR1 and NAB1 constructs, and mutation of the NAB1 binding site within the EGR1 protein. From this we conclude that EGR1 is essential to BMPR2 transcription, but that NAB1 binding to EGR1 causes it to act as a repressor.
    URI
    http://dx.doi.org/10.4236/ajmb.2011.13014
    http://hdl.handle.net/123456789/974
    Collections
    • Biology and Biotechnology [193]

    University of Embu©
    Contact Us |
    Designed by 
    Atmire NV
     

     

    Browse

    All of RepositoryCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects

    My Account

    LoginRegister

    University of Embu©
    Contact Us |
    Designed by 
    Atmire NV