Browsing Biology and Biotechnology by Title
Now showing items 89-108 of 193
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GAPDH expression as a measurement of transfection efficiency for p16INK4a gene silencing (siRNA) in senescent human diploid fibroblasts
(Scientific Research Publishing, 2012-10)Human diploid fibroblasts (HDFs) undergo a limited number of cell divisions in culture. After certain population doublings, they reach a state of irreversible growth arrest known as replicative senescence. Senescent HDFs ... -
Gene expression profiling in soybean under aluminum stress: genes differentially expressed between Al-tolerant and Al-sensitive genotypes
(Scientific Research Publishing, 2011-10)It is well documented that aluminum (Al) toxicity is the most important constraint to crop production on acid soils and soybean is one of the most Al sensitive plant species. To advance our understanding of the molecular ... -
Generation of pig primary fibroblast cells harboring defective MC4R genes by N-ethyl-N-nitrosourea mutagenesis: A gene-driven, nontransgenic approach to pig improvement
(Scientific Research Publishing, 2013-06)Transgenic pigs have been produced with the aim of further improving pigs in terms of economic and environmental traits, but these animals have not been allowed to enter the food chain. As an alternative approach to ... -
Genetic and environmental factors in skin color determination
(African Journal of Biological Sciences, 2019-04-12)The origin of skin color has been significantly a discussion of importance among human biology scientists, anthropologists and others interested in evolution of human skin color. Experience was done to chimpanzee and other ... -
Genetic diversity of hybrid durian resulted from cross breeding between Durio kutejensis and Durio zibethinus based on random amplified polymorphic DNAs (RAPDs)
(Scientific Research Publishing, 2013-07)One of the ways to improve the quality of Indonesian Durian is by utilizing germplasm diversity. Durio zibethinus is the most cultivated durian in Indonesia, whereas Durio kutejensis is a unique durian cultivar which has ... -
Genetic Diversity of Maize Accessions (Zea mays L.) Cultivated from Benin Using Microsatellites Markers
(Scientific Research Publishing, 2016-01)Maize (Zea mays L.) is the major cereal cultivated in Benin and it is important to know its genetic diversity to improve the yield. The genetic markers of important traits are evaluated in order to improve the maize ... -
Genetic variability, character association and divergence studies in sunflower (Helianthus annuus L.) for improvement in oil yield
(African Science Publications, 2021-01-01)Present experiment was conducted on 32 sunflower genotypes (11 CMS lines and 21 Restorer lines) on the basis of their diverse origin, growth habit, phenology and adaptation with an object to assess variation in oil yield ... -
Genetic Variation among Fragmented Populations of Atriplex halimus L. Using Start Codon Targeted (SCoT) and ITS1-5.8S-ITS2 Region Markers
(Scientific Research Publishing, 2016-04)Two forms of A. halimus shrubs: erect habit (A. halimus) and bushy habit shrub (A. schweinfurthii) are used naturally isolated by a considerable distance from each other and occupy the same area. To explore the effect ... -
Genetic variation may play a crucial role in non-coding RNA biogenesis
(Scientific Research Publishing, 2012-10)Transcription, post-transcriptional modification, translation, post-translational modification, DNA replication, and signaling interaction of intra- and extra- cellular components are the relevant mechanisms in gene ... -
Genome sequencing and next-generation sequence data analysis: A comprehensive compilation of bioinformatics tools and databases
(Scientific Research Publishing, 2013-04)Genomics has become a ground-breaking field in all areas of the life sciences. The advanced genomics and the development of high-throughput techniques have lately provided insight into whole-genome characterization of a ... -
Ghrelin-induced cSrc activation through constitutive nitric oxide synthase-dependent S-nitrosylation in modulation of salivary gland acinar cell inflammatory responses to Porphyromonas gingivalis
(Scientific Research Publishing, 2011-07)A peptide hormone, ghrelin, recognized for its role in the regulation of nitric oxide production has emerged as an important modulator of oral mucosal inflammatory responses to periodontopathic bacterium, P. gingivalis. ... -
Heavy metals in vegetables grown in the vicinity of Hawassa industrial zone, Ethiopia: Estimation of possible human health risks
(African Science Publications, 2021-04-05)Concentration of heavy metals (Cr, Ni, Cu, Zn, As, Cd, Pb and Hg) in cabbages and potatoes were determined and the potential human health risks due to the consumption of the vegetables have been estimated. Mean concentrations ... -
How is the biological information arranged in genome?
(Scientific Research Publishing, 2012-07)The four nucleotides (bases), A. T. G and C were sophisticatedly arranged in the structural features in a single-strand of genomic DNA, 1) reverse-complement symmetry of base or base sequences, 2) bias of four bases, 3) ... -
HPV positivity varies with technique and primer set, in formalin-fixed paraffin-embedded benign and malignant breast tissue from Malta
(Scientific Research Publishing, 2011-10)The role of human papillomavirus in breast cancer pathogenesis is an area of considerable debate following differing results from all over the world. This study analyzed paraffin embedded tissue from 19 patients with benign ... -
Identification and characterization drought tolerance of gene LEA-D11 soybean (glycine max L. Merr) based on PCR-sequencing
(Scientific Research Publishing, 2013-01)Drought is one of the most damaging abiotic stress. Different plants response differently to drought stress. Abiotic stresses such as drought induced diverse physicological and molecular responses in plants. These responses ... -
Identification and preliminary characterization of novel B3-type metallo-β-lactamases
(Scientific Research Publishing, 2013-10)Antibiotic resistance has emerged as a major global threat to human health. Among the strategies employed by pathogens to acquire resistance the use of metallo-β-lactamases (MBLs), a family of dinuclear metalloenzymes, is ... -
Identification and Quantification of Corn, Soybean and Cotton Genetically Modified by Real Time PCR
(Scientific Research Publishing, 2015-07)In order to obtain a cheaper method for quantification of transgenic events in corn, soybeans and cotton, primers for real time PCR have been developed and optimized, with fluorescent BRYT Green system. The DNA was ... -
Identification of a SSR marker (TOM-144) linked to Fusarium wilt resistance in Solanum lycopersicum
(Scientific Research Publishing, 2013-10)With the discovery of molecular markers and marker assisted selection technology, the research has entered into a new era and has made it possible to develop new and more informative PCR-based markers, including SSR, and ... -
Identification of culturable microalgae diversity in the River Nile in Egypt using enrichment media
(African Science Publications, 2021-04-05)This study aimed to morphologically identify the highest possible microalgae biodiversity in a sample collected from the River Nile using the culture-based method. Water samples were cultured on the two commonly used media ... -
Identification of ebs1, lsm6 and nup159 as suppressors of spt10 effects at ADH2 in Saccharomyces cerevisiae suggests post-transcriptional defects affect mRNA synthesis
(Scientific Research Publishing, 2012-07)Suppression of the effects of an spt10 mutation on ADH2 expression is a phenotype shared by a small number of genes whose protein products are either components of the CCR4-NOT complex required for mRNA deadenylation and ...
