Fine particle matters induce DNA damage and G2/M cell cycle arrest in human bronchial epithelial BEAS-2B cells

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dc.contributor.authorWu, J.
dc.contributor.authorShi, Y.
dc.contributor.authorAsweto, Collins O.
dc.contributor.authorFeng, L.
dc.contributor.authorYang, X.
dc.contributor.authorZhang, Y.
dc.contributor.authorHu, H.
dc.contributor.authorDuan, J.
dc.contributor.authorSun, Z.
dc.date.accessioned2018-10-11T18:27:07Z
dc.date.available2018-10-11T18:27:07Z
dc.date.issued2017-11
dc.description.abstractThere is compelling evidence that exposure to particulate matter (PM) is linked to lung tumorigenesis. However, there is not enough experimental evidence to support the specific mechanisms of PM2.5-induced DNA damage and cell cycle arrest in lung tumorigenesis. In this study, we investigated the toxic effects and molecular mechanisms of PM2.5 on bronchial epithelial (BEAS-2B) cells. PM2.5 exposure reduced cell viability and enhanced LDH activity. The cell growth curves of BEAS-2B cells decreased gradually with the increase in PM2.5 dosage. A significant increase in MDA content and a decrease in GSH-Px activity were observed. The generation of ROS was enhanced obviously, while apoptosis increased in BEAS-2B cells exposed to PM2.5 for 24 h. DNA damage was found to be more severe in the exposed groups compared with the control. For in-depth study, we have demonstrated that PM2.5 stimulated the activation of HER2/ErbB2 while significantly upregulating the expression of Ras/GADPH, p-BRAF/BRAF, p-MEK/MEK, p-ERK/ERK, and c-Myc/GADPH in a dose-dependent manner. In summary, we suggested that exposure to PM2.5 sustained the activation of HER2/ErbB2, which in turn promoted the activation of the Ras/Raf/MAPK pathway and the expression of the downstream target c-Myc. The overexpression of c-Myc may lead to G2/M arrest and aggravate the DNA damage and apoptosis in BEAS-2B after exposure to PM2.5.en_US
dc.identifier.citationEnvironmental Science and Pollution Research, 2017:1-11en_US
dc.identifier.uriDOI: 10.1007/s11356-017-0090-3
dc.identifier.urihttp://hdl.handle.net/123456789/2090
dc.language.isoenen_US
dc.publisherSpringerLinken_US
dc.subjectBEAS-2B cellsen_US
dc.subjectCell cycle arresten_US
dc.subjectCytotoxicityen_US
dc.subjectDNA damageen_US
dc.subjectPM2.5en_US
dc.subjectRas/Raf/MAPK pathwayen_US
dc.titleFine particle matters induce DNA damage and G2/M cell cycle arrest in human bronchial epithelial BEAS-2B cellsen_US
dc.typeArticleen_US
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