Browsing by Author "Wang, Changwei"

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    Advances in the development of molecular genetic tools for Mycobacterium tuberculosis
    (Elsevier, 2018-07) Chiranjibi, Chhotaray; Yaoju, Tan; Mugweru, Julius; Islam, Md Mahmudul; Hameed, H.M. Adnan; Wang, Shuai; Lu, Zhili; Wang, Changwei; Li, Xinjie; Tan, Shouyong; Liu, Jianxiong; Zhang, Tianyu
    Mycobacterium tuberculosis, a Gram-positive bacterium of great clinical relevance, is a lethal pathogen owing to its complex physiological characteristics and development of drug resistance. Several molecular genetic tools have been developed in the past few decades to study this microorganism. These tools have been instrumental in understanding how M. tuberculosis became a successful pathogen. Advanced molecular genetic tools have played a significant role in exploring the complex pathways involved in M. tuberculosis pathogenesis. Here, we review various molecular genetic tools used in the study of M. tuberculosis. Further, we discuss the applications of clustered regularly interspaced short palindromic repeat interference (CRISPRi), a novel technology recently applied in M. tuberculosis research to study target gene functions. Finally, prospective outcomes of the applications of molecular techniques in the field of M. tuberculosis genetic research are also discussed.
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    Mutation EthAW21R confers co-resistance to prothionamide and ethionamide in both Mycobacterium bovis BCG and Mycobacterium tuberculosis H37Rv
    (Dove Press, 2018-06) Mugweru, Julius; Liu, Jianxiong; Makafe, Gaelle; Chiwala, Gift; Wang, Bangxing; Wang, Changwei; Li, Xinjie; Tan, Yaoju; Yew, Wing Wai; Tan, Shouyong; Zhang, Tianyu
    Ethionamide (ETA) and prothionamide (PRO) are interchangeably used in tuberculosis (TB) chemotherapy regimens. Subtle discrepancies between biochemical and genetic information on the modes of sensitivity and resistance of isoniazid (INH) and ETA warrants further studies. We report a new mutation – EthAW21R – in Mycobacterium bovis Bacillus Calmette-Guérin that corresponds with co-resistance to both PRO and ETA, which to the best of our knowledge has not been reported before. Our findings suggest that mutation EthAW21R could be used as a marker site for testing PRO and ETA cross-resistance