Browsing by Author "Abubakar, L.U."

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    Characterization of algae oil (oilgae) and its potential as biofuel in Kenya
    (2012-05) Abubakar, L.U.; Mutie, A.M.; Kenya, Eucharia U.
    A survey of microalgae biodiversity in three Kenyan Rift valley lakes identified high oil yielding species abundantly distributed naturally. The species were cultured in BBM and BG-11 media to obtain pure clones and lipids (oil) extracted by the Bligh and Dyer method. The peak lipid content ranged from 1.5 – 10.5% of algal biomass with Chlorella species showing the highest yields (10.5%), followed by Euglena acus (5.88), Nitzschia (3.68%), Ankistrodesmus falcatus (1.58%) and Scenedesmus acuminatus (1.56 %). The algae oil extracted from Chlorella spp contained significantly higher polyunsaturated long chain fatty acids with docosatetraenoate (C22:4) and octadecatetraenoate (C18:4) to be the major components. On the other hand, algae oil from Euglena spp exhibited higher mono-unsaturated long chain fatty acids with erucate (C22:1) and eicosenoate (18:1) being the major components. This implies that algae oil from Euglena spp has a much higher degree of oxidative stability compared to Chlorella spp and has can be cultivated for biofuel.
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    The Glossina proteolytic lectin (Gpl) gene is expressed only in members of Glossina species
    (2008) Burugu, M.W.; Mbatia, B.N; Osir, E.O.; Kenya, Eucharia U.; Abubakar, L.U.
    Differentiation of bloodstream-form trypanosomes into procyclics in tsetse flies (Diptera: Glossinidae) is a crucial step in the establishment of midgut infections. A number of factors have been implicated in the transformation process, including enzymes and lectins or lectin-like molecules. Recently, Glossina proteolytic lectin (Gpl) gene, which encodes a protein with both lectin and trypsin activities has been shown to stimulate transformation of bloodstream-form trypanosomes into procyclics in vitro. Using RT-PCR, we show that the induction of Gpl gene expression by blood meal occurs only in Glossina fuscipes fuscipes Newstead, Glossina austeni Newstead, Glossina pallidipes Austen, and not in the Anopheles gambiae Giles sensu stricto, Phlebotomus duboscqi Neveu-Lemaire, Rhipicephalus appendiculatus Neumann and Stomoxys calcitrans (Linnaeus). The expression means of Gpl mRNA in G. f. fuscipes following a blood meal were significant (P , 0.05) with low expression in teneral flies and reaching a maximum between 48 and 72 h (P , 0.05), suggesting time-dependent regulation of the transcription. The expression of the Gpl gene was significantly lower (P , 0.05) in G. f. fuscipes fed on blood meal infected with Trypanosoma brucei brucei as compared with G. f. fuscipes fed on uninfected blood meal. This suggests some form of interaction of T. b. brucei or the parasite products with Gpl within the tsetse midgut leading to down-regulation of the Gpl gene. Additionally, refractory G. f. fuscipes expressed higher (P , 0.05) transcript abundance than the susceptible G. pallidipes.
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    The Glossina proteolytic lectin (Gpl) gene is expressed only in members of Glossina species
    (2007-12) Burugu, M.W.; Mbatia, B.N; Osir, E.O.; Kenya, Eucharia U.; Abubakar, L.U.
    Differentiation of bloodstream-form trypanosomes into procyclics in tsetse flies (Diptera: Glossinidae) is a crucial step in the establishment of midgut infections. A number of factors have been implicated in the transformation process, including enzymes and lectins or lectin-like molecules. Recently, Glossina proteolytic lectin (Gpl) gene, which encodes a protein with both lectin and trypsin activities has been shown to stimulate transformation of bloodstream-form trypanosomes into procyclics in vitro. Using RT-PCR, we show that the induction of Gpl gene expression by blood meal occurs only in Glossina fuscipes fuscipes Newstead, Glossina austeni Newstead, Glossina pallidipes Austen, and not in the Anopheles gambiae Giles sensu stricto, Phlebotomus duboscqi Neveu-Lemaire, Rhipicephalus appendiculatus Neumann and Stomoxys calcitrans (Linnaeus). The expression means of Gpl mRNA in G. f. fuscipes following a blood meal were significant (P < 0.05) with low expression in teneral flies and reaching a maximum between 48 and 72 h (P < 0.05), suggesting time-dependent regulation of the transcription. The expression of the Gpl gene was significantly lower (P < 0.05) in G. f. fuscipes fed on blood meal infected with Trypanosoma brucei brucei as compared with G. f. fuscipes fed on uninfected blood meal. This suggests some form of interaction of T. b. brucei or the parasite products with Gpl within the tsetse midgut leading to down-regulation of the Gpl gene. Additionally, refractory G. f. fuscipes expressed higher (P < 0.05) transcript abundance than the susceptible G. pallidipes