Biology and Biotechnology

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Browsing Biology and Biotechnology by Author "Adjanohoun, Adolphe"

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    Genetic Diversity of Maize Accessions (Zea mays L.) Cultivated from Benin Using Microsatellites Markers
    (Scientific Research Publishing, 2016-01) Salami, Hafiz A.; Sika, Kamirou C.; Padonou, Wilfrid; Aly, Djima; Yallou, Chabi; Adjanohoun, Adolphe; Kotchoni, Simeon; Baba-Moussa, Lamine
    Maize (Zea mays L.) is the major cereal cultivated in Benin and it is important to know its genetic diversity to improve the yield. The genetic markers of important traits are evaluated in order to improve the maize inbred lines. The aim of this study was to evaluate the genetic diversity of Benin’s maize accessions by SSR marker. Thus, one hundred eighty seven maize accessions from three areas (South, Center and North) were analyzed using three SSR markers. A total of 227 polymorphic bands were produced and showed high genetic diversity (Shannon index = 0.51). The polymorphic information content (PIC) values for the SSR loci ranged from 0.58 to 0.81, with an average of 0.71. Genetic distance-based UPGMA dendrogram showed a genetic differentiation between accessions and they were grouped into four clusters in each area. This work provides necessary information that can be used not only to improve the maize production and conservation but also to better manage genetic species resources in Benin.
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    A simple and efficient seed-based approach to induce callus production from B73 maize genotype
    (Scientific Research Publishing, 2012-10) Kotchoni, Simeon O.; Noumavo, Pacôme A.; Adjanohoun, Adolphe; Russo, Daniel P.; Dell’Angelo, John; W. Gachomo, Emma; Baba-Moussa, Lamine
    The wild type maize genotype, B73, is not amenable for callus production and an efficient protocol for B73 maize callus induction has never been reported up-to-date. Scientific efforts in producing B73 maize callus using all known callus inducible media have been unsatisfactory. Here we developed and described an efficient protocol for callus induction from B73 maize seedlings. The protocol is based on well known callus inducible media CM4C where we have sequentially subtracted some chemical compounds and added some new compounds mediating cell proliferations. This newly described protocol was able to induce callus production in a wide range of crop species including rice and soybean. We found that cell proliferation factors, NAA (auxin analog) and 2,4 D (auxin influx carrier) were not only very crucial but required for positive B73 maize callus induction. The absence of one or the other will lead to the failure of B73 maize callus production. The well known CM4C callus induction composition lacks NAA. Our findings will advance genetic studies of maize mutants generated from B73 genotype background.