Browsing Biology and Biotechnology by Issue Date
Now showing items 81-100 of 193
-
Potential effects of ultraviolet-C radiation on the mole rats (Spalax leucodon) hematological values
(Scientific Research Publishing, 2013-10)In this study, the effect of ultraviolet-C (UVC) radiation on hematologic parameters of mole rats (Spalax leucodon) was studied. Ten mole rats of both sexes, weighing 150-200 g, were used. The rats were exposed to 8 hours ... -
Characterization of winged bean (Psophocarpus tetragonolobus (L.) DC.) based on molecular, chemical and physiological parameters
(Scientific Research Publishing, 2013-10)Winged bean (Psophocarpus tetragonolobus (L.) DC.) is a potential legume crop of the tropics with high protein and oil content in the seeds. Analysis of the mutual genotypic relationships among twenty four genotypes of P. ... -
A method to synthesize cDNA constructs by homology based recombination cloning
(Scientific Research Publishing, 2014-01)We introduce a homology-based recombination approach for generating a cDNA construct. This method depends on amplifying several exon fragments and their fusions by the homology-based recombination. This method provides a ... -
Unusual metallo-β-lactamases may constitute a new subgroup in this family of enzymes
(Scientific Research Publishing, 2014-01)Metallo-β-lactamases (MBLs) are a family of Zn2+-dependent enzymes that have contributed strongly to the emergence and spread of antibiotic resistance. Novel members as well as variants of existing members of this family ... -
Diagnostic and prognostic value of plasma level of microRNA-92a in acute myeloid leukemia
(Scientific Research Publishing, 2014-01)Background: MicroRNAs (miRNAs) are short noncoding RNAs of ~21 to 23 nucleotides in length that post-transcriptionally regulate mRNA expression. Highthroughput methodologies have shown deregulated miRNA expression in an ... -
Polysaccharides as initiators of nucleic acid polymerization
(Scientific Research Publishing, 2014-10)Quantum-chemical calculations and in vitro experiments have demonstrated that polysaccharides are able to adsorb mononucleotides due to the formation of hydrogen bonds. The subsequent dehydration of polysaccharide-nucleotide ... -
Inteins—A Focus on the Biotechnological Applications of Splicing-Promoting Proteins
(Scientific Research Publishing, 2015-03)The main aim of this mini-review is to illustrate strategies and industrial applications based on inteins (INTErnal proteINS), which belong to a class of autocatalytic enzymes that are able to perform a catalytic reaction ... -
Identification and Quantification of Corn, Soybean and Cotton Genetically Modified by Real Time PCR
(Scientific Research Publishing, 2015-07)In order to obtain a cheaper method for quantification of transgenic events in corn, soybeans and cotton, primers for real time PCR have been developed and optimized, with fluorescent BRYT Green system. The DNA was ... -
TRPC3 and TRPC6 Contribute to the Pathogenesis of Hypertension
(Scientific Research Publishing, 2015-10)Recently studies found that TRPC3 and TRPC6 played an important role in cardiovascular disease. Hypertension, as a cardiovascular disease causing the highest morbidity and mortality, has close relationship with the ... -
Extracellular Micro-RNAs in Health and Disease: Basic Science, Biogenesis and Release
(Scientific Research Publishing, 2016-01)Small non-protein coding micro-RNAs are regularly exported out of cells, both in health and disease. More than ninety percent of extracellular miRNAs are associated with lower-molecular-mass complexes bound to Argonaute ... -
Genetic Diversity of Maize Accessions (Zea mays L.) Cultivated from Benin Using Microsatellites Markers
(Scientific Research Publishing, 2016-01)Maize (Zea mays L.) is the major cereal cultivated in Benin and it is important to know its genetic diversity to improve the yield. The genetic markers of important traits are evaluated in order to improve the maize ... -
Association of XbaI GLUT1 Polymorphism with Susceptibility to Type 2 Diabetes Mellitus and Diabetic Nephropathy
(Scientific Research Publishing, 2016-03)Objectives: Diabetic nephropathy (DN) is one of the chronic microangiopathic complications of type 2 diabetes (T2DM) and has become the most frequent cause of end-stage renal disease. The XbaI polymorphism in the glucose ... -
Genetic Variation among Fragmented Populations of Atriplex halimus L. Using Start Codon Targeted (SCoT) and ITS1-5.8S-ITS2 Region Markers
(Scientific Research Publishing, 2016-04)Two forms of A. halimus shrubs: erect habit (A. halimus) and bushy habit shrub (A. schweinfurthii) are used naturally isolated by a considerable distance from each other and occupy the same area. To explore the effect ... -
Quantitative Gene Expression of Peroxidase, Polyphenoloxidase and Catalase as Molecular Markers for Resistance against Ralstonia solanacearum
(Scientific Research Publishing, 2016-04)Brown rot or bacterial wilt of potato caused by Ralstonia solanacearum, is an economically important disease. Potato, cv. Nicola, was found to be relatively highly resistant to the infection with R. solanacearum and ... -
Constitutive and Secretory Expression of the AiiA in Pichia pastoris Inhibits Amorphophallus konjac Soft Rot Disease
(Scientific Research Publishing, 2016-04)Amorphophallus konjac is an important economic crop widely cultivated in Southeast Asia and Africa. However, A. konjac is seriously infected by soft rot pathogen. The endocellular acyl homoserine lactonase (AiiA) which ... -
E lectrospun fibers for wound healing and treatment of hyperglycemia: A review
(Academic Journals, 2016-06-22)The use of electrospun fibers in diabetic wound healing assays represents an extremely important topic for investigation. Electrospun fibers have been applied in the immobilization of drugs, allowing sustained and controlled ... -
Super-Enhancement and Control of Amh Expression
(Scientific Research Publishing, 2016-07)In previous work it was shown that mutation of site 1 in the downstream enhancer sequence (DE) led to ablation of enhancement. Mutation of the Wilms tumour factor element (Wt), situated in the Amh promoter between the tata ... -
Anaplerosis in cancer: Another step beyond the warburg effect
(Scientific Research Publishing, 2016-10)Biosynthesis is up-regulated in tumors and thus the demand for anabolic intermediates is increased. The metabolic routes providing the building blocks for macromolecules are thus a very attractive target as they are not ... -
The Potential of DNA Barcode-Based Delineation Using Seven Putative Candidate Loci of the Plastid Region in Inferring Molecular Diversity of Cowpea at Sub-Species Level
(Scientific Research Publishing, 2016-10)The novelty and suitability of the mitochondrial gene CO1 in DNA barcoding as a reliable identification tool in animal species are undisputed. This is attributed to its standardized sequencing segment of the mitochondrial ... -
Expression Analysis of WRKY Transcription Factor Genes in Response to Abiotic Stresses in Horsegram (Macrotyloma uniflorum (Lam.) Verdc.)
(Scientific Research Publishing, 2016-10)Drought and salt stress are two major environmental constraints that limit the productivity of agriculture crops worldwide. WRKY transcription factors are the plant-specific transcription factors that regulate several ...