A method to synthesize cDNA constructs by homology based recombination cloning
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Date
2014-01
Authors
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Journal ISSN
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Publisher
Scientific Research Publishing
Abstract
We introduce a homology-based recombination approach for generating a cDNA construct. This method depends on amplifying several exon fragments and their fusions by the homology-based recombination. This method provides a way to generate the cDNA sequence of any gene without any need for its mRNA. The paper describes the strategy by assembling cDNA of the MYB1 and MYB2 genes of Arabidopsis thaliana.
Description
Keywords
Homology Based Recombination Cloning, cDNA Assembly
Citation
American Journal of Molecular Biology, 2014, 4, 16-19