Browsing Biology and Biotechnology by Issue Date
Now showing items 61-80 of 193
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Identification and characterization drought tolerance of gene LEA-D11 soybean (glycine max L. Merr) based on PCR-sequencing
(Scientific Research Publishing, 2013-01)Drought is one of the most damaging abiotic stress. Different plants response differently to drought stress. Abiotic stresses such as drought induced diverse physicological and molecular responses in plants. These responses ... -
Bax promoter G(-248)A polymorphism in a Turkish clinical breast cancer patients: A case-control study
(Scientific Research Publishing, 2013-01)Bax is an important protein involved in apoptotic process. Mutations of the Bax gene are known to af- fect protein expression and function. A promotor polymorphism G(-248)A in the 5’UTR of Bax gene may alter regulation of ... -
The molecular regulatory effect of intracerebroventricular thymulin on endotoxin-mediated NF-kB nuclear translocation and activation in vivo
(Scientific Research Publishing, 2013-01)The nuclear factor-kB (NF-kB) is one member of a ubiquitously expressed family of Rel-related transcription factors that serve as critical regulators of proinflammatory genes. The immunomodulatory potential of thymulin and ... -
Research of total levels on DNA methylation in plant based on HPLC analysis
(Scientific Research Publishing, 2013-04)HPLC analysis is important for determination of total level on DNA methylation in plants. It can be used to help characterise epigenetic changes during growth, development and stress. HPLC methods have been optimised for ... -
Genome sequencing and next-generation sequence data analysis: A comprehensive compilation of bioinformatics tools and databases
(Scientific Research Publishing, 2013-04)Genomics has become a ground-breaking field in all areas of the life sciences. The advanced genomics and the development of high-throughput techniques have lately provided insight into whole-genome characterization of a ... -
Breeding of NHase hyper-producing Rhodococcus ruber strain LUV30-06 and verification of mutants by RAPD
(Scientific Research Publishing, 2013-04)A nitrile hydratase (NHase) hyper-producing Rhodococcus ruber strain LUV30-06 was bred by mutagenization on the starting strain CGMCC3090 with ultraviolet irradiation and lithium chloride. The NHase activity of the strain ... -
Plasmid instability when the hsp60 gene promoter is used to express the protective non-toxic fragment B of the diphtheria toxin in recombinant BCG
(Scientific Research Publishing, 2013-04)The genetic modification of the live attenuated Mycobacterium bovis BCG to deliver a protective Corynebacterium diphtheriae antigen in vivo could be a safer and less costly alternative to the new and more expensive DTP ... -
Effect of SP600125 on proliferation of embryonic stem cell
(Scientific Research Publishing, 2013-04)SP600125 is an inhibitor of c-Jun NH2-terminal kinase (JNK), which plays a fundamental role in regulating animal development. Using SP600125 to deal with the mouse embryonic stem cells, it is revealed that the number of ... -
1,25-Dihydroxyvitamin D3 effects on the regulation of the insulin receptor gene in the hind limb muscle and heart of streptozotocin-induced diabetic rats
(Scientific Research Publishing, 2013-04)In the present study, we examine the effects of the treatment with 1,25-dihydroxyvitamin D3 [150 IU/Kg (3.75 μg/Kg) once a day, for 15 days] to non-diabetic and streptozotocin-induced diabetic rats. The results indicate ... -
Generation of pig primary fibroblast cells harboring defective MC4R genes by N-ethyl-N-nitrosourea mutagenesis: A gene-driven, nontransgenic approach to pig improvement
(Scientific Research Publishing, 2013-06)Transgenic pigs have been produced with the aim of further improving pigs in terms of economic and environmental traits, but these animals have not been allowed to enter the food chain. As an alternative approach to ... -
Determination and inheritance of phytic acid as marker in diverse genetic group of bread wheat
(Scientific Research Publishing, 2013-07)Phytic acid (Myo-inositol 1,2,3,4,5,6 hexa-kisphophate) is a storage form of phosphorus and can accumulate to the levels as high as 35% in the wheat kernel. Phytic acid acts as an inhibitor for macronutrients as well as ... -
Quantitative detection of Streptococcus mutans from saliva using FTATM elute cards and real-time polymerase chain reaction
(Scientific Research Publishing, 2013-07)Dental caries is a localized, transmissible, pathological infection process that ends up in the destruction of hard dental tissue. Numerous reports have shown the close relationship between salivary levels of Streptococcus ... -
Genetic diversity of hybrid durian resulted from cross breeding between Durio kutejensis and Durio zibethinus based on random amplified polymorphic DNAs (RAPDs)
(Scientific Research Publishing, 2013-07)One of the ways to improve the quality of Indonesian Durian is by utilizing germplasm diversity. Durio zibethinus is the most cultivated durian in Indonesia, whereas Durio kutejensis is a unique durian cultivar which has ... -
Interaction between the Wilms tumour factor-1 element in the promoter of Amh and a downstream enhancer is required for a strong expression of the gene in pre-pubertal sertoli cells
(Scientific Research Publishing, 2013-07)Amh (anti-Müllerian hormone) is a single copy gene which is expressed strongly in Sertoli cells in the foetal testis and participates in the onset of sexual differentiation. Its promoter driving the expression of a reporter ... -
Identification and preliminary characterization of novel B3-type metallo-β-lactamases
(Scientific Research Publishing, 2013-10)Antibiotic resistance has emerged as a major global threat to human health. Among the strategies employed by pathogens to acquire resistance the use of metallo-β-lactamases (MBLs), a family of dinuclear metalloenzymes, is ... -
Comparative study on some selected species of Ocimum genus on free radical scavenging activity and hepatoprotective activity against CCl4 induced intoxication in rats
(Scientific Research Publishing, 2013-10)In this study, we compared the in vitro antioxidant property among the selected Ocimum species (O. sanctum, O. americanum, O. basilicum and O. gratissimum) and hepatoprotective activities of their extracts against CCl4 ... -
Functional reconstruction of bovine P450scc steroidogenic system in Escherichia coli
(Scientific Research Publishing, 2013-10)Mammalian cytochrome P450scc enzyme system catalyzes the initial step in steroid hormone biosynthesis—cholesterol hydroxylation followed by cleavage of the side-chain to yield pregnenolone. This system consists of three ... -
TLR5 involvement in attenuated IL-8 production in nuclear decorin silenced oral mucosal dysplastic keratinocytes and squamous cell carcinoma
(Scientific Research Publishing, 2013-10)Head and neck cancer is one of the most prevalent cancers in the world. Roughly half of these malignancies originate from oral mucosa and constitute Oral squamous cell carcinomas. Despite many advances in diagnostic and ... -
Identification of a SSR marker (TOM-144) linked to Fusarium wilt resistance in Solanum lycopersicum
(Scientific Research Publishing, 2013-10)With the discovery of molecular markers and marker assisted selection technology, the research has entered into a new era and has made it possible to develop new and more informative PCR-based markers, including SSR, and ... -
The origin of biological information and programmed protein synthesis
(Scientific Research Publishing, 2013-10)Biological information is one of the most important characteristics of life, and it enables life to evolve to higher complexity and adapt to the environment by mutation and natural selection. However, the origin of this ...