Browsing Biology and Biotechnology by Title
Now showing items 81-100 of 193
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Explaining the benefits of human genome patent
(African Journal of Biological Sciences, 2019-04-12)Gene patenting has been facing backlash for decades now. Even Michael Crichton, science fiction novelist, as joined the bandwagon speaking against gene patent.1 Such hostility towards patents, the professional interests ... -
Expression Analysis of WRKY Transcription Factor Genes in Response to Abiotic Stresses in Horsegram (Macrotyloma uniflorum (Lam.) Verdc.)
(Scientific Research Publishing, 2016-10)Drought and salt stress are two major environmental constraints that limit the productivity of agriculture crops worldwide. WRKY transcription factors are the plant-specific transcription factors that regulate several ... -
Expression and subcellular localization of a novel gene Bm-X of the silkworm, Bombyx mori
(Scientific Research Publishing, 2012-07)According to the large-scale sequencing of cDNA library from silkworm pupae, the cDNA of a novel gene with blank research background was identified and temporarily named Bm-X. The length of this cDNA is 778 bp. We obtained ... -
Expression of the transcription factor Xvent-2 in Xenopus laevis embryogenesis
(Scientific Research Publishing, 2012-04)Till now the transcription factor Xvent-2 has been studied in Xenopus embryos only by the mRNA testing. We use immunochemical methods for testing of the Xvent-2 protein and gradient-centrifugation methods for estimation ... -
Extracellular Micro-RNAs in Health and Disease: Basic Science, Biogenesis and Release
(Scientific Research Publishing, 2016-01)Small non-protein coding micro-RNAs are regularly exported out of cells, both in health and disease. More than ninety percent of extracellular miRNAs are associated with lower-molecular-mass complexes bound to Argonaute ... -
Frequently cutting restriction enzymes: Clearing the fog to see the ends
(Scientific Research Publishing, 2013-01)Telomeres, which are found at the ends of eukaryotic chromosomes, are composed of tandem arrays of repetitive sequences and safeguard genomic stability. Previous studies have revealed that telomeric repeats are also present ... -
The full length PtSRP (Pisolithus tinctorius symbiosis related protein) fungal mRNA encodes a potential marker of ectomycorrhiza formation
(Scientific Research Publishing, 2012-07)The Pisolithus tinctorius symbiosis related protein expressed sequence tag (EST PtSRP) was previously identified in the first hours of the interaction between the fungus Pisolithus tinctorius and sweet chestnut Castanea ... -
Functional reconstruction of bovine P450scc steroidogenic system in Escherichia coli
(Scientific Research Publishing, 2013-10)Mammalian cytochrome P450scc enzyme system catalyzes the initial step in steroid hormone biosynthesis—cholesterol hydroxylation followed by cleavage of the side-chain to yield pregnenolone. This system consists of three ... -
GAPDH expression as a measurement of transfection efficiency for p16INK4a gene silencing (siRNA) in senescent human diploid fibroblasts
(Scientific Research Publishing, 2012-10)Human diploid fibroblasts (HDFs) undergo a limited number of cell divisions in culture. After certain population doublings, they reach a state of irreversible growth arrest known as replicative senescence. Senescent HDFs ... -
Gene expression profiling in soybean under aluminum stress: genes differentially expressed between Al-tolerant and Al-sensitive genotypes
(Scientific Research Publishing, 2011-10)It is well documented that aluminum (Al) toxicity is the most important constraint to crop production on acid soils and soybean is one of the most Al sensitive plant species. To advance our understanding of the molecular ... -
Generation of pig primary fibroblast cells harboring defective MC4R genes by N-ethyl-N-nitrosourea mutagenesis: A gene-driven, nontransgenic approach to pig improvement
(Scientific Research Publishing, 2013-06)Transgenic pigs have been produced with the aim of further improving pigs in terms of economic and environmental traits, but these animals have not been allowed to enter the food chain. As an alternative approach to ... -
Genetic and environmental factors in skin color determination
(African Journal of Biological Sciences, 2019-04-12)The origin of skin color has been significantly a discussion of importance among human biology scientists, anthropologists and others interested in evolution of human skin color. Experience was done to chimpanzee and other ... -
Genetic diversity of hybrid durian resulted from cross breeding between Durio kutejensis and Durio zibethinus based on random amplified polymorphic DNAs (RAPDs)
(Scientific Research Publishing, 2013-07)One of the ways to improve the quality of Indonesian Durian is by utilizing germplasm diversity. Durio zibethinus is the most cultivated durian in Indonesia, whereas Durio kutejensis is a unique durian cultivar which has ... -
Genetic Diversity of Maize Accessions (Zea mays L.) Cultivated from Benin Using Microsatellites Markers
(Scientific Research Publishing, 2016-01)Maize (Zea mays L.) is the major cereal cultivated in Benin and it is important to know its genetic diversity to improve the yield. The genetic markers of important traits are evaluated in order to improve the maize ... -
Genetic variability, character association and divergence studies in sunflower (Helianthus annuus L.) for improvement in oil yield
(African Science Publications, 2021-01-01)Present experiment was conducted on 32 sunflower genotypes (11 CMS lines and 21 Restorer lines) on the basis of their diverse origin, growth habit, phenology and adaptation with an object to assess variation in oil yield ... -
Genetic Variation among Fragmented Populations of Atriplex halimus L. Using Start Codon Targeted (SCoT) and ITS1-5.8S-ITS2 Region Markers
(Scientific Research Publishing, 2016-04)Two forms of A. halimus shrubs: erect habit (A. halimus) and bushy habit shrub (A. schweinfurthii) are used naturally isolated by a considerable distance from each other and occupy the same area. To explore the effect ... -
Genetic variation may play a crucial role in non-coding RNA biogenesis
(Scientific Research Publishing, 2012-10)Transcription, post-transcriptional modification, translation, post-translational modification, DNA replication, and signaling interaction of intra- and extra- cellular components are the relevant mechanisms in gene ... -
Genome sequencing and next-generation sequence data analysis: A comprehensive compilation of bioinformatics tools and databases
(Scientific Research Publishing, 2013-04)Genomics has become a ground-breaking field in all areas of the life sciences. The advanced genomics and the development of high-throughput techniques have lately provided insight into whole-genome characterization of a ... -
Ghrelin-induced cSrc activation through constitutive nitric oxide synthase-dependent S-nitrosylation in modulation of salivary gland acinar cell inflammatory responses to Porphyromonas gingivalis
(Scientific Research Publishing, 2011-07)A peptide hormone, ghrelin, recognized for its role in the regulation of nitric oxide production has emerged as an important modulator of oral mucosal inflammatory responses to periodontopathic bacterium, P. gingivalis. ... -
Heavy metals in vegetables grown in the vicinity of Hawassa industrial zone, Ethiopia: Estimation of possible human health risks
(African Science Publications, 2021-04-05)Concentration of heavy metals (Cr, Ni, Cu, Zn, As, Cd, Pb and Hg) in cabbages and potatoes were determined and the potential human health risks due to the consumption of the vegetables have been estimated. Mean concentrations ...