Characterization, Enzymatic Activity, and Secondary Metabolites of Fungal Isolates from Lake Sonachi in Kenya

dc.contributor.authorNdwigah, Ireri F.
dc.contributor.authorBogai, Hamadi I.
dc.contributor.authorWanyoike, Wanjiru
dc.contributor.authorMwirichia, Romano K.
dc.date.accessioned2015-12-09T10:13:34Z
dc.date.available2015-12-09T10:13:34Z
dc.date.issued2015-03
dc.description.abstractThe soda lakes of Kenya provide an extreme environment where diverse groups of microorganisms thrive. They are characterized by great variation in temperature, halophillic and alkaliphilic- extreme conditions. Lake Sonachi has been the study site for this research. The study sort to isolate, characterize and identify fungi, screen for potential exo-enzymes and secondary metabolites production that may be of industrial application. Malt extract agar was used in the isolation of fungi and six (6) isolates were recovered. Inhibition zones were used to measure the enzymatic and antimicrobial activity of the isolates. GC-MC analysis was done on the filtrates extracted from the fungi to identify secondary metabolites. Molecular characterization of the 18s rDNA was done using fungal primers and sequencing PCR products.s .Phylogenetic tree was inferred using neighbor- joining method. The fungal isolates were alighned to diferrent genera, Acrimonies sp., Scopulariopsissp, Verticilium sp. Fusariumsp and Paecilomyces sp. The fungal isolates produce different types of enzymes (cellulases, proteases, pectinases and lipases) and metabolites (acids, ketones, quinones, alcohols, esters etc) .Antimicrobial assay showed that most of the fungal isolates produced inhibition zones ranging from 0.1 to 4mm, an indication of presence of compounds with antimicrobial activity against most of the test organisms, E.coli, B. subtilis, S.aureasetc, used in this study. Results indicate that Lake Sonachi, a soda lake has fungal species that are capable of producing enzymes and metabolites with antimicrobial activity.en_US
dc.identifier.citationJournal of Pharmacy and Biological Sciences Volume 10, Issue 2 Ver. 1 , pp. 65-76en_US
dc.identifier.issn2278-3008
dc.identifier.issnISSN:2319-7676
dc.identifier.urihttp://hdl.handle.net/123456789/568
dc.language.isoenen_US
dc.subjectCharacterizationen_US
dc.subjectEnzymatic activityen_US
dc.subjectAntimicrobial activityen_US
dc.subjectSecondary metaboliteen_US
dc.titleCharacterization, Enzymatic Activity, and Secondary Metabolites of Fungal Isolates from Lake Sonachi in Kenyaen_US
dc.typeArticleen_US
Files
Original bundle
Now showing 1 - 1 of 1
Loading...
Thumbnail Image
Name:
Mwirichia_Characterization, Enzymatic Activity, and Secondary Metabolites of Fungal Isolates from Lake Sonachi in Kenya.pdf
Size:
645.5 KB
Format:
Adobe Portable Document Format
Description:
Full text
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description: