Effect of 4% glycerol and low aeration on result of expression in Escherichia coli of Cin3 and three Venturia inaequalis EST’s recombinant proteins
| dc.contributor.author | Al-Samarrai, Taha H. | |
| dc.contributor.author | T. Jones, William | |
| dc.contributor.author | Harvey, Dawn | |
| dc.contributor.author | A. Kirk, Christopher | |
| dc.contributor.author | Templtone, M. | |
| dc.date.accessioned | 2016-10-25T06:36:39Z | |
| dc.date.available | 2016-10-25T06:36:39Z | |
| dc.date.issued | 2013-01 | |
| dc.description.abstract | The phytopathogenic fungus Venturia inaequalis causes scab of apple. Once this fungus penetrates the plant surface, it forms a specialized body called a stroma between the inner cuticle surface and the epidermal cell wall. A novel Venturia inaequalis 5704 (Cin3) and three expressed sequence tags (ESTs); 38, 6987, and 4010 are strongly up-regulated in the early stages of infection. The CIN3 and three ESTs using two vectors pMAL-c2 and pET 21 were expressed in Escherichia coli. Recombinant proteins expression, solubility and yields were analyzed. 38, 5704 (Cin3) and 6987 re- combinant proteins were expressed in soluble form and while 4010 was expressed in inclusion bodies. Re- solution on native-PAGE, the recombinant proteins; 38, 5704 (Cin3), 6987 were shown to be present in dimmer, tetramer and polymer. A method was de- veloped, consisting of induction of expression at va- rious temperatures, and using enriched broth with 4% glycerol together with slow shaking, led to a decrease in concentration of nascent polypeptide and production of soluble recombinant proteins of; 38, 5704 (Cin3), 6987 and 4010. Resolution on native- PAGE, the recombinant proteins were shown to be present as monomer. | en_US |
| dc.identifier.citation | American Journal of Molecular Biology, 2013, 3, 1-9 | en_US |
| dc.identifier.uri | http://hdl.handle.net/123456789/1027 | |
| dc.language.iso | en | en_US |
| dc.publisher | Scientific Research Publishing | en_US |
| dc.subject | Venturia inaequalis | en_US |
| dc.subject | Expressed Sequence Tag (ESTs) | en_US |
| dc.subject | Phytopathogenic Fungus | en_US |
| dc.subject | Appressorium | en_US |
| dc.subject | A Stroma | en_US |
| dc.title | Effect of 4% glycerol and low aeration on result of expression in Escherichia coli of Cin3 and three Venturia inaequalis EST’s recombinant proteins | en_US |
| dc.type | Article | en_US |