Purification of Dengue Virus Particles by One-Step Ceramic Hydroxyapatite Chromatography

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dc.contributor.authorKurosawa, Yae
dc.contributor.authorSaito, Maiko
dc.contributor.authorKobayashi, Shintaro
dc.contributor.authorOkuyama, Tsuneo
dc.date.accessioned2018-06-25T06:06:14Z
dc.date.available2018-06-25T06:06:14Z
dc.date.issued2012-08
dc.description.abstractDengue virus type 2 ThNH7/93 retained infectious activity after purification by ceramic hydroxyapatite chromatography. Dengue virus type 2 culture fluid was loaded onto the ceramic hydroxyapatite column and eluted with a linear gradient of sodium phosphate buffer. Culture fluid and protein contaminants derived from host cells were eluted initially, followed by elutions of dsDNA, and then dengue virus type 2. The recoveries of dengue virus type 2 were 64 ± 14% (n = 11) in the hemagglutination (HA) test and 60% (n = 2) determined by focus assay for viral infectivity. This protocol was highly reproducible, simple, rapid, and appears applicable to other virus species such as influenza virus, Japanese encephalitis virus and adenovirusen_US
dc.description.sponsorshipDepartment of Virology, Institute of Tropical Medicine, Nagasaki University, Japan. Department of Virology, National Institute of Infectious Diseases, Japan.en_US
dc.identifier.citationWorld Journal of Vaccines, 2012, 2, 155-160en_US
dc.identifier.urihttp://dx.doi.org/10.4236/wjv.2012.23020
dc.identifier.urihttp://hdl.handle.net/123456789/1535
dc.language.isoenen_US
dc.publisherScientific Researchen_US
dc.subjectCeramic Hydroxyapatiteen_US
dc.subjectVirus Purificationen_US
dc.subjectDengue Virusen_US
dc.subjectVirus Particleen_US
dc.subjectVirionen_US
dc.titlePurification of Dengue Virus Particles by One-Step Ceramic Hydroxyapatite Chromatographyen_US
dc.typeArticleen_US
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