Inteins—A Focus on the Biotechnological Applications of Splicing-Promoting Proteins

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dc.contributor.authorMiraula, Manfredi
dc.contributor.authorEnculescu, Charmaine
dc.contributor.authorMitić, Natasa
dc.contributor.authorSchenk, Gerhard
dc.date.accessioned2016-06-20T06:27:30Z
dc.date.available2016-06-20T06:27:30Z
dc.date.issued2015-03
dc.description.abstractThe main aim of this mini-review is to illustrate strategies and industrial applications based on inteins (INTErnal proteINS), which belong to a class of autocatalytic enzymes that are able to perform a catalytic reaction on a single substrate. However, since practical applications of inteins are strongly guided by a detailed understanding of their biological mechanisms and functions, the first part of this review will thus briefly discuss the physiological roles of inteins, describing what is currently known about their mechanisms of action. In the second part, specific biotechnological applications of inteins will be outlined (i.e. their use for (i) the purification of recombinant proteins, (ii) the cyclization of proteins and (iii) the production of seleno-proteins), paying attention to both potential strengths and weaknesses of this technology.en_US
dc.identifier.citationAmerican Journal of Molecular Biology, 2015, 5, 42-56en_US
dc.identifier.urihttp://dx.doi.org/10.4236/ajmb.2015.52005
dc.identifier.urihttp://hdl.handle.net/123456789/826
dc.language.isoenen_US
dc.publisherScientific Research Publishingen_US
dc.subjectInteinen_US
dc.subjectProtein Purificationen_US
dc.subjectTagged Proteinen_US
dc.subjectCyclizationen_US
dc.subjectSelenoproteinen_US
dc.titleInteins—A Focus on the Biotechnological Applications of Splicing-Promoting Proteinsen_US
dc.typeArticleen_US
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