The Novel Pyruvated Glucogalactan Sulfate Isolated from the Red Seaweed, Hypnea pannosa
dc.contributor.author | Tako, Masakuni | |
dc.contributor.author | Ohtoshi, Rintaro | |
dc.contributor.author | Kinjyo, Kazutaka | |
dc.contributor.author | Uech, Shuntoku | |
dc.date.accessioned | 2016-07-25T12:27:52Z | |
dc.date.available | 2016-07-25T12:27:52Z | |
dc.date.issued | 2016-06 | |
dc.description.abstract | The polysaccharide was isolated from Hypnea pannosa which was grown in Okinawa, Japan. The yield of the polysaccharide was 17.2%, and the total carbohydrates, pyruvic acid, sulfuric acid and ash contents were 55.2%, 3.8%, 35.2% and 24.3%, respectively. 3,6-Anhydro-α-D-galactose, β-D-galactose, α-D-galactose and D-glucose were identified by liquid and thin-layer chromatography. Fourier transform infrared (FTIR) spectra of the polysaccharide resembled that of ι-carrageenan. From the 1H- and 13C-NMR spectra, 1,3-linked β-D-galactose, 1,4-linked anhydro-α-D-galactose, 1,4-linked α-D-galactose, 1,4-linked β-D-glucose and pyruvic acid (carboxyl acetal, methyl proton and methyl carbon) were assigned. Methylation analysis revealed terminal D-galactose 0.1 mol), 1,4-linked D-glucose (1.0 mol) and 1,2,3,4,6-linked D-galactose (3.7 mol) for native polysaccharide, and terminal D-galactose, 1,4-linked D-galactose (1.9 mol), 1,4-linked D-glucose (1.0 mol), 1,3- linked D-galactose (1.7 mol), and 1,3,4,6-linked D-galactose (0.3 mol) which substituted with pyruvate group at 4 and 6 positions for desulfated polysaccharide. The polysaccharide was the novel pyruvated glucogalactan sulfate, the structure of which was proposed. | en_US |
dc.identifier.uri | http://dx.doi.org/10.4236/abc.2016.63010 | |
dc.identifier.uri | http://hdl.handle.net/123456789/906 | |
dc.language.iso | en | en_US |
dc.publisher | Scientific Research Publishing | en_US |
dc.relation.ispartofseries | Advances in Biological Chemistry , 2016, 6, 114- 125; | |
dc.subject | Hypnea pannosa | en_US |
dc.subject | Pyruvated Glucogalactan Sulfate | en_US |
dc.subject | 1 H- and 13 C-NMR Analysis | en_US |
dc.subject | Methylation Analysis | en_US |
dc.subject | Chemical Structure | en_US |
dc.title | The Novel Pyruvated Glucogalactan Sulfate Isolated from the Red Seaweed, Hypnea pannosa | en_US |
dc.type | Article | en_US |