A method to synthesize cDNA constructs by homology based recombination cloning

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dc.contributor.authorVerma, Neetu
dc.contributor.authorBurma, Pradeep K.
dc.date.accessioned2016-10-17T08:17:00Z
dc.date.available2016-10-17T08:17:00Z
dc.date.issued2014-01
dc.description.abstractWe introduce a homology-based recombination approach for generating a cDNA construct. This method depends on amplifying several exon fragments and their fusions by the homology-based recombination. This method provides a way to generate the cDNA sequence of any gene without any need for its mRNA. The paper describes the strategy by assembling cDNA of the MYB1 and MYB2 genes of Arabidopsis thaliana.en_US
dc.identifier.citationAmerican Journal of Molecular Biology, 2014, 4, 16-19en_US
dc.identifier.urihttp://dx.doi.org/10.4236/ajmb.2014.41003
dc.identifier.urihttp://hdl.handle.net/123456789/958
dc.language.isoenen_US
dc.publisherScientific Research Publishingen_US
dc.subjectHomology Based Recombination Cloningen_US
dc.subjectcDNA Assemblyen_US
dc.titleA method to synthesize cDNA constructs by homology based recombination cloningen_US
dc.typeArticleen_US
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