HPV positivity varies with technique and primer set, in formalin-fixed paraffin-embedded benign and malignant breast tissue from Malta

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dc.contributor.authorMallia, Roderick
dc.contributor.authorMangion, Jean-Paul
dc.contributor.authorCamenzuli, Christian
dc.contributor.authorCassar, Annalisse
dc.contributor.authorCacciottolo, Paul
dc.contributor.authorCauchi, John
dc.contributor.authorBorg, Joseph
dc.contributor.authorAli, Sarfraz
dc.contributor.authorSchembri-Wismayer, Pierre
dc.date.accessioned2016-10-19T06:26:14Z
dc.date.available2016-10-19T06:26:14Z
dc.date.issued2011-10
dc.description.abstractThe role of human papillomavirus in breast cancer pathogenesis is an area of considerable debate following differing results from all over the world. This study analyzed paraffin embedded tissue from 19 patients with benign or malignant breast disease. Standard PCR with GP5+/6+ and FAP primers, proved negative in all cases. However, qRT-PCR using the SF10 primer set detected HPV DNA in 2 out of these samples. Whilst our data does not support a major role for HPV in the pathogenesis of breast disease in Malta, it definitely does not exclude it totally. This study also clearly shows that the method of analysis is one of the variables resulting in the range of differing results witnessed worldwide in this area of study.en_US
dc.identifier.citationAmerican Journal of Molecular Biology, 2011, 1, 183-18en_US
dc.identifier.urihttp://dx.doi.org/10.4236/ajmb.2011.13018
dc.identifier.urihttp://hdl.handle.net/123456789/980
dc.language.isoenen_US
dc.publisherScientific Research Publishingen_US
dc.subjectHuman Papillomavirus (HPV)en_US
dc.subjectBreast Canceren_US
dc.subjectBenignen_US
dc.subjectqRT-PCRen_US
dc.titleHPV positivity varies with technique and primer set, in formalin-fixed paraffin-embedded benign and malignant breast tissue from Maltaen_US
dc.typeArticleen_US
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