EGR1 is essential for transcriptional regulation of BMPR2

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dc.contributor.authorGaddipati, Radhika
dc.contributor.authorWest, James D.
dc.contributor.authorLoyd, James E.
dc.contributor.authorBlackwell, Thomas
dc.contributor.authorLane, Kirsten A.
dc.contributor.authorLane, Nicole M.
dc.contributor.authorLane, Kirk B.
dc.date.accessioned2016-10-18T09:32:19Z
dc.date.available2016-10-18T09:32:19Z
dc.date.issued2011-10
dc.description.abstractIn this study, RLM-RACE was used to identify the transcriptional start site 387 bp upstream of the translational start. Evolutionarily conserved transcription factor binding sites were identified, and a series of luciferase reporter constructs driven by BMPR2 promoter elements used to determine their functional relevance. We found the promoter area from 983 bp to 90 bp upstream of the transcriptional start gave maximal activity, greater than longer constructs, with an area between 570 bp and 290 bp upstream of the transcriptional start containing an important repressor element. To characterize this repressor, we used a combination of EMSA, mutation of the EGR1 binding site, transfection with EGR1 and NAB1 constructs, and mutation of the NAB1 binding site within the EGR1 protein. From this we conclude that EGR1 is essential to BMPR2 transcription, but that NAB1 binding to EGR1 causes it to act as a repressor.en_US
dc.identifier.citationAmerican Journal of Molecular Biology, 2011, 1, 131-139en_US
dc.identifier.urihttp://dx.doi.org/10.4236/ajmb.2011.13014
dc.identifier.urihttp://hdl.handle.net/123456789/974
dc.language.isoenen_US
dc.publisherScientific Research Publishingen_US
dc.subjectTranscriptionen_US
dc.subjectBMPR2 Regulationen_US
dc.subjectEGR1en_US
dc.subjectNAB1en_US
dc.subjectPulmonary Hypertensionen_US
dc.titleEGR1 is essential for transcriptional regulation of BMPR2en_US
dc.typeArticleen_US
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