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A method to synthesize cDNA constructs by homology based recombination cloning
(Scientific Research Publishing, 2014-01)
We introduce a homology-based recombination approach for generating a cDNA construct. This method depends on amplifying several exon fragments and their fusions by the homology-based recombination. This method provides a ...
Unusual metallo-β-lactamases may constitute a new subgroup in this family of enzymes
(Scientific Research Publishing, 2014-01)
Metallo-β-lactamases (MBLs) are a family of Zn2+-dependent enzymes that have contributed strongly to the emergence and spread of antibiotic resistance. Novel members as well as variants of existing members of this family ...
Diagnostic and prognostic value of plasma level of microRNA-92a in acute myeloid leukemia
(Scientific Research Publishing, 2014-01)
Background: MicroRNAs (miRNAs) are short noncoding RNAs of ~21 to 23 nucleotides in length that post-transcriptionally regulate mRNA expression. Highthroughput methodologies have shown deregulated miRNA expression in an ...
Polysaccharides as initiators of nucleic acid polymerization
(Scientific Research Publishing, 2014-10)
Quantum-chemical calculations and in vitro experiments have demonstrated that polysaccharides are able to adsorb mononucleotides due to the formation of hydrogen bonds. The subsequent dehydration of polysaccharide-nucleotide ...