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Generation of pig primary fibroblast cells harboring defective MC4R genes by N-ethyl-N-nitrosourea mutagenesis: A gene-driven, nontransgenic approach to pig improvement
(Scientific Research Publishing, 2013-06)
Transgenic pigs have been produced with the aim of further improving pigs in terms of economic and environmental traits, but these animals have not been allowed to enter the food chain. As an alternative approach to ...
Determination and inheritance of phytic acid as marker in diverse genetic group of bread wheat
(Scientific Research Publishing, 2013-07)
Phytic acid (Myo-inositol 1,2,3,4,5,6 hexa-kisphophate) is a storage form of phosphorus and can accumulate to the levels as high as 35% in the wheat kernel. Phytic acid acts as an inhibitor for macronutrients as well as ...
Characterization of the role of Smu1 in nuclear localization of splicing factors in the mammalian temperature-sensitive mutant
(Scientific Research Publishing, 2013-01)
A temperature-sensitive (ts) mutant of the CHO-K1 cell line, tsTM18, grows at 340C but not at 390C. Smu1 is the gene responsible for ts defects of tsTM18 cells. Previously, we found that the Smu1 ts defect altered the ...
Quantitative detection of Streptococcus mutans from saliva using FTATM elute cards and real-time polymerase chain reaction
(Scientific Research Publishing, 2013-07)
Dental caries is a localized, transmissible, pathological infection process that ends up in the destruction of hard dental tissue. Numerous reports have shown the close relationship between salivary levels of Streptococcus ...
Genetic diversity of hybrid durian resulted from cross breeding between Durio kutejensis and Durio zibethinus based on random amplified polymorphic DNAs (RAPDs)
(Scientific Research Publishing, 2013-07)
One of the ways to improve the quality of Indonesian Durian is by utilizing germplasm diversity. Durio zibethinus is the most cultivated durian in Indonesia, whereas Durio kutejensis is a unique durian cultivar which has ...
Frequently cutting restriction enzymes: Clearing the fog to see the ends
(Scientific Research Publishing, 2013-01)
Telomeres, which are found at the ends of eukaryotic chromosomes, are composed of tandem arrays of repetitive sequences and safeguard genomic stability. Previous studies have revealed that telomeric repeats are also present ...
Using Bayesian and Eigen approaches to study spatial genetic structure of Moroccan and Syrian durum wheat landraces
(Scientific Research Publishing, 2013-01)
The Mediterranean durum wheat landraces are genetically diverse and important sources for improving resistance to abiotic and biotic stresses and developing adapted and productive durum wheat varieties in the Mediterranean ...
Identification and characterization drought tolerance of gene LEA-D11 soybean (glycine max L. Merr) based on PCR-sequencing
(Scientific Research Publishing, 2013-01)
Drought is one of the most damaging abiotic stress. Different plants response differently to drought stress. Abiotic stresses such as drought induced diverse physicological and molecular responses in plants. These responses ...
Comparative study on some selected species of Ocimum genus on free radical scavenging activity and hepatoprotective activity against CCl4 induced intoxication in rats
(Scientific Research Publishing, 2013-10)
In this study, we compared the in vitro antioxidant property among the selected Ocimum species (O. sanctum, O. americanum, O. basilicum and O. gratissimum) and hepatoprotective activities of their extracts against CCl4 ...
Breeding of NHase hyper-producing Rhodococcus ruber strain LUV30-06 and verification of mutants by RAPD
(Scientific Research Publishing, 2013-04)
A nitrile hydratase (NHase) hyper-producing Rhodococcus ruber strain LUV30-06 was bred by mutagenization on the starting strain CGMCC3090 with ultraviolet irradiation and lithium chloride. The NHase activity of the strain ...