Wang, ShiweiWang, MinWang, Qinghui2016-10-252016-10-252013-04American Journal of Molecular Biology, 2013, 3, 108-114http://dx.doi.org/10.4236/ajmb.2013.32015http://hdl.handle.net/123456789/1036A nitrile hydratase (NHase) hyper-producing Rhodococcus ruber strain LUV30-06 was bred by mutagenization on the starting strain CGMCC3090 with ultraviolet irradiation and lithium chloride. The NHase activity of the strain LUV30-06 was increased by 21.99% (3881.3 U/ml), as compared with that of R. ruber CGMCC3090 (3181.4 U/ml). The mutant strain UV30-06 has been proved genetically stable with higher NHase activity in seven successive subcultures as well as random amplified polymorphic DNA analysis (RAPD).enRhodococcus ruberNHaseMutagenesisRandom Amplified Polymorphic DNAArticle