Horie, MasanoriIwahashi, Hitoshi2018-07-122018-07-122014-04J Phys Chem Biophys 2014, Vol 4(2): 1392161-0398DOI: 10.4172/2161-0398.1000139http://hdl.handle.net/123456789/1837Recently, many in vitro studies evaluating the effects of nanoparticles on cellular physiology have been reported. In in vitro systems, the nano-objects induce not only primary effects but also confounding (artificial) effects. Investigations into the physiological and pathological effects induced in cells by in vitro exposure to nano-objects may be confounded by the specific physical and chemical properties of the objects. For example, protein adsorption from the culture media to the surfaces of nano-objects can essentially starve the cells. In addition, certain nanoparticles can release metal ions into cell culture or bioassay reagents. The protein adsorption and metal ion release by the nano-objects can interfere with ELISA and LDH assays, producing inaccurate results. Moreover, unstable or non-homogenous suspensions of nano-objects can result in imprecise in vitro evaluations of nano-objects. For accurate in vitro testing of nanoparticles, we should consider the effects of these three important properties of nanosuspensions: protein adsorption, metal ion release, and suspension stability.enNano-objectNanosuspensionAdsorptionMetal ionSuspension stabilityArticle