Pathogenic Fungal Diversity in Onions (Allium cepa) from Different Growing Regions of Kenya
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Date
2019
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University of Embu
Abstract
Onions in storage are affected by different species of fungal pathogens causing rots
leading to huge monetary losses. In addition, the fungal pathogens are a health risk to
onion consumers. We seek to study the diversity and abundance of post-harvest fungal
pathogens affecting different varieties of onions in Kenyan markets. Samples of “red”
and “white” bulb onions were obtained from three markets, i.e., Bungoma, Naivasha
and Nyeri, and brought into the laboratory for fungal pathogen analyses. In the lab,
samples were crashed in a pestle and mortar, inoculated in Potato dextrose agar and
incubated in an oven set at 28
for seven days for growth of fungal pathogens.
Thereafter, the pathogens were identified using colour and morphological
characteristics. Fungal species diversity and abundance were subjected to descriptive
and inferential methods of statistical analysis. Seven fungal species were isolated from
both red and white onions across the three markets. Aspergillus niger and Fusarium sp.
were isolated from both onion cultivars. Candida tropicalis and Saccharomyces
cerevisiae were isolated from red onions but not white onion, while R. stolonifera and
Scopulariopsis brevicaulis were isolated from white but not red onions. We discuss
these results on post-harvest fungal pathogens in onions, and propose management
measures in this document. Onions in storage are affected by different species of fungal pathogens causing rots
leading to huge monetary losses. In addition, the fungal pathogens are a health risk to
onion consumers. We seek to study the diversity and abundance of post-harvest fungal
pathogens affecting different varieties of onions in Kenyan markets. Samples of “red”
and “white” bulb onions were obtained from three markets, i.e., Bungoma, Naivasha
and Nyeri, and brought into the laboratory for fungal pathogen analyses. In the lab,
samples were crashed in a pestle and mortar, inoculated in Potato dextrose agar and
incubated in an oven set at 28
for seven days for growth of fungal pathogens.
Thereafter, the pathogens were identified using colour and morphological
characteristics. Fungal species diversity and abundance were subjected to descriptive
and inferential methods of statistical analysis. Seven fungal species were isolated from
both red and white onions across the three markets. Aspergillus niger and Fusarium sp.
were isolated from both onion cultivars. Candida tropicalis and Saccharomyces
cerevisiae were isolated from red onions but not white onion, while R. stolonifera and
Scopulariopsis brevicaulis were isolated from white but not red onions. We discuss
these results on post-harvest fungal pathogens in onions, and propose management
measures in this document.
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University of Embu, Undergraduate Projects 2019