Comparative cytomorphometry of red blood cells of some fishes

Abstract

This study aims to compare the size of the red blood cells of different freshwater fishes to gain knowledge of their activity. In this study, 11 different freshwater fishes of four different order like Siluriformes, Cypriniformes, Perciformes, and Osteoglossiformes were selected. For analyses of cytomorphometry of blood cells, blood was collected from caudal vein, and blood smear was prepared at the site of collection. In order to obtain size of different blood cell types, 30 cells of each cell type for each fish were photographed and dimensions of cell-like length and breadth were measured using Microscope Eyepiece Digital Camera (CatCam130 – 1.3 Mega Pixel (MP), Code No. CC130, Catalyst Biotech, Maharashtra, India, attached to Hund Wetzlar Microscope GmbH, Wetzlar-Nauborn, Germany) and computer. This study confirms the cytomorphometry of red blood cells differ significantly at p< 0.001 concerning sex and species. The study will help in diagnosis which in turn will accelerate production of fishes.