Antagonistic conflict of Trichoderma harzianum against fruit rots pathogens

Abstract

Present work was investigated the study of infected fruits and its pathogens and checked the antagonistic activity with Trichoderma harzianum. Ficus carica was to be found highly mycotoxic due to more pathogens followed by Manilkara zapota and Punica granatum Antagonistic potentials of T. harzianum was tested against fruit rots pathogens in vitro conditions. Dual culture experiment was conducted for pathogens and T. harzianum and data revealed that, the percent inhibition of test fungi proved to be more than 50% antagonism over control. In case of Fusarium oxysporum (83.34%) showed highest antagonistic activity followed by others. It was found highly inhibited the radial growth over control. In case of F. oxysporum, overgrew beyond 80% (R1-scale) and Phytophthora parasitica, Cladosporim cladosporioides, Penicillium digitatum, Mucor sp, Fusarium sp and Geotrichum candidum overgrew beyond 70% (R2-scale). In case of Bipolaris cactivora, overgrow on bioagent (R5-scale) and Aspergillus flavus, A. tamari and Rhizopus nigricans were found the bioagent and test fungi contact point after inoculation (R4-scale). The results of this study identify as promising biological control agents for further testing against rots disease on fruits.