| dc.contributor.author | Wu, Jinping | |
| dc.contributor.author | Jiao, Zhenbiao | |
| dc.contributor.author | Guo, Fengling | |
| dc.contributor.author | Chen, Leifu | |
| dc.contributor.author | Ding, Zili | |
| dc.contributor.author | Qiu, Zhengming | |
| dc.date.accessioned | 2016-06-20T05:40:02Z | |
| dc.date.available | 2016-06-20T05:40:02Z | |
| dc.date.issued | 2016-04 | |
| dc.identifier.citation | American Journal of Molecular Biology, 2016, 6, 79-87 | en_US |
| dc.identifier.uri | http://dx.doi.org/10.4236/ajmb.2016.62009 | |
| dc.identifier.uri | http://hdl.handle.net/123456789/818 | |
| dc.description.abstract | Amorphophallus konjac is an important economic crop widely cultivated in Southeast Asia and
Africa. However, A. konjac is seriously infected by soft rot pathogen. The endocellular acyl homoserine
lactonase (AiiA) which is generated by Bacillus species has inhibitory effect on soft rot pathogen
through disrupting the signal molecules (N-acylhomoserine lactones, AHL) of their Quorum
Sensing system. The aim of our study is to obtain recombinant yeast which produces AiiA
protein. The recombinant yeast Pichia pastoris GS115 was constructed to constitutive expression
of the AiiA gene. The results of reverse transcript PCR analysis showed that the AiiA gene was expressed
successfully in the yeast. Proteins extracted from YPDS showed the highest inhibition efficacy
to E. carotovora compared with the other two mediums (YPD and LB) under tested conditions. | en_US |
| dc.language.iso | en | en_US |
| dc.publisher | Scientific Research Publishing | en_US |
| dc.subject | Amorphophallus konjac | en_US |
| dc.subject | Acyl Homoserine Lactonase | en_US |
| dc.subject | Pichia pastoris | en_US |
| dc.subject | Fusion-Expression | en_US |
| dc.title | Constitutive and Secretory Expression of the AiiA in Pichia pastoris Inhibits Amorphophallus konjac Soft Rot Disease | en_US |
| dc.type | Article | en_US |
