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Research of total levels on DNA methylation in plant based on HPLC analysis
(Scientific Research Publishing, 2013-04)
HPLC analysis is important for determination of total level on DNA methylation in plants. It can be used to help characterise epigenetic changes during growth, development and stress. HPLC methods have been optimised for ...
Genome sequencing and next-generation sequence data analysis: A comprehensive compilation of bioinformatics tools and databases
(Scientific Research Publishing, 2013-04)
Genomics has become a ground-breaking field in all areas of the life sciences. The advanced genomics and the development of high-throughput techniques have lately provided insight into whole-genome characterization of a ...
Effect of 4% glycerol and low aeration on result of expression in Escherichia coli of Cin3 and three Venturia inaequalis EST’s recombinant proteins
(Scientific Research Publishing, 2013-01)
The phytopathogenic fungus Venturia inaequalis causes scab of apple. Once this fungus penetrates the plant surface, it forms a specialized body called a stroma between the inner cuticle surface and the epidermal cell wall. ...
Identification and preliminary characterization of novel B3-type metallo-β-lactamases
(Scientific Research Publishing, 2013-10)
Antibiotic resistance has emerged as a major global threat to human health. Among the strategies employed by pathogens to acquire resistance the use of metallo-β-lactamases (MBLs), a family of dinuclear metalloenzymes, is ...
Generation of pig primary fibroblast cells harboring defective MC4R genes by N-ethyl-N-nitrosourea mutagenesis: A gene-driven, nontransgenic approach to pig improvement
(Scientific Research Publishing, 2013-06)
Transgenic pigs have been produced with the aim of further improving pigs in terms of economic and environmental traits, but these animals have not been allowed to enter the food chain. As an alternative approach to ...
Determination and inheritance of phytic acid as marker in diverse genetic group of bread wheat
(Scientific Research Publishing, 2013-07)
Phytic acid (Myo-inositol 1,2,3,4,5,6 hexa-kisphophate) is a storage form of phosphorus and can accumulate to the levels as high as 35% in the wheat kernel. Phytic acid acts as an inhibitor for macronutrients as well as ...
Characterization of the role of Smu1 in nuclear localization of splicing factors in the mammalian temperature-sensitive mutant
(Scientific Research Publishing, 2013-01)
A temperature-sensitive (ts) mutant of the CHO-K1 cell line, tsTM18, grows at 340C but not at 390C. Smu1 is the gene responsible for ts defects of tsTM18 cells. Previously, we found that the Smu1 ts defect altered the ...
Quantitative detection of Streptococcus mutans from saliva using FTATM elute cards and real-time polymerase chain reaction
(Scientific Research Publishing, 2013-07)
Dental caries is a localized, transmissible, pathological infection process that ends up in the destruction of hard dental tissue. Numerous reports have shown the close relationship between salivary levels of Streptococcus ...
Genetic diversity of hybrid durian resulted from cross breeding between Durio kutejensis and Durio zibethinus based on random amplified polymorphic DNAs (RAPDs)
(Scientific Research Publishing, 2013-07)
One of the ways to improve the quality of Indonesian Durian is by utilizing germplasm diversity. Durio zibethinus is the most cultivated durian in Indonesia, whereas Durio kutejensis is a unique durian cultivar which has ...
Frequently cutting restriction enzymes: Clearing the fog to see the ends
(Scientific Research Publishing, 2013-01)
Telomeres, which are found at the ends of eukaryotic chromosomes, are composed of tandem arrays of repetitive sequences and safeguard genomic stability. Previous studies have revealed that telomeric repeats are also present ...