• Login
    View Item 
    •   Repository
    • Open Access Articles
    • Open Access Journals
    • Agriculture
    • View Item
    •   Repository
    • Open Access Articles
    • Open Access Journals
    • Agriculture
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Effect of sorghum (Sorghum bicolor L. Moench) grain conditions on occurrence of mycotoxin‑producing fungi

    Thumbnail
    View/Open
    Full text (1.190Mb)
    Date
    2015
    Author
    Kange, Alex M.
    Cheruiyot, Erick K.
    Ogendo, Joshua O.
    Arama, Peter F.
    Metadata
    Show full item record
    Abstract
    Background: Sorghum is a staple cereal crop that is well adapted to arid and semi-arid lands (ASALs). It has a potential of assuring food security and livelihoods in the ASALs. The objective of this study was to determine the effect of sorghum grain conditions on occurrence of mycotoxin-producing fungi. Methods: Two kilograms of sorghum grains were sampled from the breeder’s crop at Egerton University research field and at a farmer’s field at Kampi Ya Moto. Sorghum was sampled at dough stage and at physiological maturity. Sorghum grains sampled at dough stage were divided into three sets. Set one was immediately examined for fungi; set two was sun dried for 21 h and set three was stored for a fortnight before being examined for fungi. Grains were plated on potato dextrose agar medium and incubated at 25 ± 2 °C for 7 days. Fungal colonies growing were sub-cultured and identified using a microscope and a standard mycological catalogue based on micro- and macromorphological features. Result: Identified fungi were Aspergillus, Penicillium and Fusarium species. Aflatoxins were detected in 37 samples using reverse-phased HPLC at a wavelength of 365 nm. The aflatoxin B1, B2, G1 and G2 were detected in 10.81, 5.41, 18.92 and 32.43 % of the samples, respectively. There was no aflatoxin detected on 32.42 % samples. These results would contribute to reduced risk of mycotoxin-producing fungi in sorghum grain, minimize grain losses and improve grain quality among smallholder farmers in sorghum growing areas. Conclusion: Aspergillus, Fusarium and Penicillium species of fungi do occur in sorghum grain both in the field and in the store.
    URI
    http://hdl.handle.net/123456789/744
    Collections
    • Agriculture [96]

    University of Embu copyright ©  2021
    Contact us | Send Feedback
    Library ER 
    Atmire NV
     

     

    Browse

    All of RepositoryCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects

    My Account

    LoginRegister

    University of Embu copyright ©  2021
    Contact us | Send Feedback
    Library ER 
    Atmire NV