Show simple item record

dc.contributor.authorKaruri, H.W.
dc.contributor.authorAmata, R.
dc.contributor.authorAmugune, C.N.
dc.contributor.authorWaturu, C.N.
dc.date.accessioned2015-07-14T12:53:30Z
dc.date.available2015-07-14T12:53:30Z
dc.date.issued2013
dc.identifier.citationSpanish Journal of Agricultural Research, vol. 11 pp.968-979.en_US
dc.identifier.urihttp://hdl.handle.net/123456789/191
dc.description.abstractThe quality of decomposing plant materials may affect the soil community structure. The aim of the study was to determine the impact of decomposing Bt cotton and its isoline on soil nematode diversity. Bt cotton (06Z604D), isoline (99M03) and HART 89M (local non-Bt cotton cultivar) were planted for two seasons in a completely randomized block design in a confined field trial at Mwea, Kenya. After harvest the plant material was incorporated into soil and the nematode diversity was determined. The presence of Bt protein was evaluated using ELISA and insect bioassays. Abundance of bacteria feeding nematodes was significantly (p < 0.05) high but to a smaller extent in the Bt cotton plots (53.7% and 52% in the first and second season respectively) than in isoline (42.8% and 45% in the first and second season respectively). Insect bioassays detected Bt protein in the Bt cotton plots during the entire decomposition period in both seasons. There were no significant differences in nematode trophic groups composition between isoline and HART 89M. The effect of Cry2Ab2 and Cry1Ac protein in decomposing Bt cotton litter on soil nematodes was minimal. The study provides a basis for future studies on the impact of genetically engineered plants on soil nematodes in Kenyan agroecosystems.en_US
dc.language.isoenen_US
dc.subjectBacillus thuringiensisen_US
dc.subjectbiosafetyen_US
dc.subjectHelicoverpa armigeraen_US
dc.subjectnematodesen_US
dc.titleNematode diversity in soil from a field trial with decomposing Bt cotton expressing Cry1Ac and Cry2Ab2 proteinen_US
dc.typeArticleen_US


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record