Breeding of NHase hyper-producing Rhodococcus ruber strain LUV30-06 and verification of mutants by RAPD
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Date
2013-04
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Scientific Research Publishing
Abstract
A nitrile hydratase (NHase) hyper-producing Rhodococcus ruber strain LUV30-06 was bred by mutagenization on the starting strain CGMCC3090 with ultraviolet irradiation and lithium chloride. The NHase activity of the strain LUV30-06 was increased by 21.99% (3881.3 U/ml), as compared with that of R. ruber CGMCC3090 (3181.4 U/ml). The mutant strain UV30-06 has been proved genetically stable with higher NHase activity in seven successive subcultures as well as random amplified polymorphic DNA analysis (RAPD).
Description
Keywords
Rhodococcus ruber, NHase, Mutagenesis, Random Amplified Polymorphic DNA
Citation
American Journal of Molecular Biology, 2013, 3, 108-114