| dc.contributor.author | Wang, Shiwei | |
| dc.contributor.author | Wang, Min | |
| dc.contributor.author | Wang, Qinghui | |
| dc.date.accessioned | 2016-10-25T14:15:35Z | |
| dc.date.available | 2016-10-25T14:15:35Z | |
| dc.date.issued | 2013-04 | |
| dc.identifier.citation | American Journal of Molecular Biology, 2013, 3, 108-114 | en_US |
| dc.identifier.uri | http://dx.doi.org/10.4236/ajmb.2013.32015 | |
| dc.identifier.uri | http://hdl.handle.net/123456789/1036 | |
| dc.description.abstract | A nitrile hydratase (NHase) hyper-producing Rhodococcus ruber strain LUV30-06 was bred by mutagenization on the starting strain CGMCC3090 with ultraviolet irradiation and lithium chloride. The NHase activity of the strain LUV30-06 was increased by 21.99% (3881.3 U/ml), as compared with that of R. ruber CGMCC3090 (3181.4 U/ml). The mutant strain UV30-06 has been proved genetically stable with higher NHase activity in seven successive subcultures as well as random amplified polymorphic DNA analysis (RAPD). | en_US |
| dc.language.iso | en | en_US |
| dc.publisher | Scientific Research Publishing | en_US |
| dc.subject | Rhodococcus ruber | en_US |
| dc.subject | NHase | en_US |
| dc.subject | Mutagenesis | en_US |
| dc.subject | Random Amplified Polymorphic DNA | en_US |
| dc.title | Breeding of NHase hyper-producing Rhodococcus ruber strain LUV30-06 and verification of mutants by RAPD | en_US |
| dc.type | Article | en_US |
