dc.contributor.author | F. Ufot, Usenobong | |
dc.contributor.author | Akpanabiatu, Monday I. | |
dc.date.accessioned | 2016-10-25T06:00:03Z | |
dc.date.available | 2016-10-25T06:00:03Z | |
dc.date.issued | 2012-10 | |
dc.identifier.citation | American Journal of Molecular Biology, 2012, 2, 359-370 | en_US |
dc.identifier.uri | http://dx.doi.org/10.4236/ajmb.2012.24037 | |
dc.identifier.uri | http://hdl.handle.net/123456789/1023 | |
dc.description.abstract | Manganese peroxidases (MnPs) are interesting enzymes in protein engineering, aimed at maximizing industrial bioprocesses such as lignin degradation and biofuel production. cDNA of the secreted short-type of MnP from Phlebia radiata (Pr-MnP3) has been successfully engineered and amplified by polymerase chain reaction (PCR). Five mutant genes (E40H, E44H, E40H/E44H, D186H and D186N) of recombinant Phlebia radiata MnP3 (rPr-MnP3) were generated. The wild-type and the mutant genes were expressed in Escherichia coli (W3110 strain) and the resultant body proteins were lysed, purified and refolded into active enzymes. 6% - 7% recovery of pure and fully active rPr-MnP3 for wild-type and mutants were obtained and the availability of rPr-MnP3 enzymes will greatly facilitate its structure-function relationships studies. rPr-MnP3 mass was characterised using SDS-PAGE and MALDI-TOF mass spectrometry. Molecular weight of both the wild-type and mutant rPr-MnP3 enzymes was approximately 36 kDa. This describes the spectral characterization of the wild-type and mutant rPr-MnP3 enzymes with are very close similarities; substantially high spin haem enzymes. Therefore we report the engineering, cloning, expression, refolding/activation of MnP3 genes and preliminary characterization of the wild-type and mutant Phlebia radiata MnP3 enzymes. | en_US |
dc.language.iso | en | en_US |
dc.publisher | Scientific Research Publishing | en_US |
dc.subject | Phlebia Radiata | en_US |
dc.subject | Peroxidase | en_US |
dc.subject | Manganese Peroxidase | en_US |
dc.subject | Over-Expression | en_US |
dc.subject | Recombinant | en_US |
dc.title | An engineered Phlebia radiata manganese peroxidase: expression, refolding, purification and preliminary characterization | en_US |
dc.type | Article | en_US |