dc.contributor.author | Lv, Zhengbing | |
dc.contributor.author | Liu, Yunlong | |
dc.contributor.author | Chen, Ying | |
dc.contributor.author | Li, Bo | |
dc.contributor.author | Liu, Lili | |
dc.contributor.author | Chen, Jian | |
dc.contributor.author | Nie, Zuoming | |
dc.contributor.author | Sheng, Qing | |
dc.contributor.author | Yu, Wei | |
dc.contributor.author | Zhang, Yaozhou | |
dc.date.accessioned | 2016-10-24T13:36:13Z | |
dc.date.available | 2016-10-24T13:36:13Z | |
dc.date.issued | 2012-07 | |
dc.identifier.citation | American Journal of Molecular Biology, 2012, 2, 224-23 | en_US |
dc.identifier.uri | http://dx.doi.org/10.4236/ajmb.2012.23023 | |
dc.identifier.uri | http://hdl.handle.net/123456789/1007 | |
dc.description.abstract | According to the large-scale sequencing of cDNA library from silkworm pupae, the cDNA of a novel gene with blank research background was identified and temporarily named Bm-X. The length of this cDNA is 778 bp. We obtained its ORF for further study by bioinformatics analysis. It is 444 bp and encodes 147 amino acid residues, with a predicted molecular weight (MW) of 16.4 kD and an isoelectric point (pI) of 3.69. In this study, we successfully constructed a recombinant plasmid pET-28a(+)-Bm-X and expressed it in Escherichia coli. We used the fusion protein rBm-X which purified by Niaffinity chromatography to produce polyclonal antibodies against Bm-X for Western blot analysis. The analysis revealed that Bm-X was expressed in the larval midgut, the epidermis and the silk gland. In addition, the subcellular localization analysis of silkworm ovary epithelial cells (BmN cells) showed that Bm-X protein was located both in cytoplasm and nucleus, and the signal was stronger in cytoplasm than in nucleus. Our findings indicate that Bm-X gene is a novel species-specificity gene and its expression product can be detected in tissues of the fifth silkworm instar larvae and BmN cells. | en_US |
dc.language.iso | en | en_US |
dc.publisher | Scientific Research Publishing | en_US |
dc.subject | Bombyx Mori | en_US |
dc.subject | Novel Gene | en_US |
dc.subject | Expression | en_US |
dc.subject | Subcellular Localization | en_US |
dc.subject | Bm-X | en_US |
dc.title | Expression and subcellular localization of a novel gene Bm-X of the silkworm, Bombyx mori | en_US |
dc.type | Article | en_US |